RRC ID 3338
Author Takeda O, Takechi S, Ito S, Omori H, Katoh T, Yamaguchi T.
Title Effects of phenyl derivatives of dihydropyrazines with ability to generate radical species on Escherichia coli.
Journal Biol Pharm Bull
Abstract Phenyl-substituted dihydropyrazines (Ph-DHPs) are derivatives of 2,3-dihydro-5,6-dimethylpyrazine (Me-DHP). Upon the addition of Cu(2+), Me-DHP inhibits the growth of Escherichia coli by generating hydroxyl and carbon-centered radicals that cause DNA strand breakage. Here, we investigated the toxic effect of Ph-DHPs in several DNA repair-deficient or detoxifying enzyme-deficient mutant strains. Ph-DHPs caused cytotoxic and genotoxic damage, but, in a sodA sodB strain, the effects in the presence or absence of Cu(2+) were different than those of Me-DHP. Our results suggest that the action of the generated superoxide anion in the interior side of the cell is remarkable.
Volume 30(9)
Pages 1663-7
Published 2007-9-1
DOI 10.1248/bpb.30.1663
PII JST.JSTAGE/bpb/30.1663
PMID 17827717
MeSH Aminolevulinic Acid / metabolism Copper / pharmacology DNA / drug effects DNA / genetics DNA Repair / drug effects Electron Spin Resonance Spectroscopy Escherichia coli K12 / drug effects Escherichia coli K12 / genetics Escherichia coli K12 / metabolism* Liver Neoplasms / chemically induced Liver Neoplasms / pathology Mutation Phenyl Ethers / chemistry Phenyl Ethers / metabolism Pyrazines / chemistry Pyrazines / pharmacology* Tetrazolium Salts / metabolism
IF 1.54
Times Cited 14
Prokaryotes E. coli ME8079(AB1157) ME8087(AB2463) ME8080(AB1885)