Reference - Detail
|Author||Takeda O, Takechi S, Ito S, Omori H, Katoh T, Yamaguchi T.|
|Title||Effects of phenyl derivatives of dihydropyrazines with ability to generate radical species on Escherichia coli.|
|Journal||Biol Pharm Bull|
Phenyl-substituted dihydropyrazines (Ph-DHPs) are derivatives of 2,3-dihydro-5,6-dimethylpyrazine (Me-DHP). Upon the addition of Cu(2+), Me-DHP inhibits the growth of Escherichia coli by generating hydroxyl and carbon-centered radicals that cause DNA strand breakage. Here, we investigated the toxic effect of Ph-DHPs in several DNA repair-deficient or detoxifying enzyme-deficient mutant strains. Ph-DHPs caused cytotoxic and genotoxic damage, but, in a sodA sodB strain, the effects in the presence or absence of Cu(2+) were different than those of Me-DHP. Our results suggest that the action of the generated superoxide anion in the interior side of the cell is remarkable.
|MeSH||Aminolevulinic Acid / metabolism Copper / pharmacology DNA / drug effects DNA / genetics DNA Repair / drug effects Electron Spin Resonance Spectroscopy Escherichia coli K12 / drug effects Escherichia coli K12 / genetics Escherichia coli K12 / metabolism* Liver Neoplasms / chemically induced Liver Neoplasms / pathology Mutation Phenyl Ethers / chemistry Phenyl Ethers / metabolism Pyrazines / chemistry Pyrazines / pharmacology* Tetrazolium Salts / metabolism|
|WOS Category||PHARMACOLOGY & PHARMACY|
|Prokaryotes E. coli||ME8079(AB1157) ME8087(AB2463) ME8080(AB1885)|