論文 - 詳細
| RRC ID | 33383 |
|---|---|
| 著者 | DeLaFuente A, Cisneros E, Talavera A. |
| タイトル | Restriction end-converting vectors with tandem repeated multiple cloning sites. |
| ジャーナル | Gene |
| Abstract |
The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles. |
| 巻・号 | 139(1) |
| ページ | 83-6 |
| 公開日 | 1994-2-11 |
| DOI | 10.1016/0378-1119(94)90527-4 |
| PII | 0378-1119(94)90527-4 |
| PMID | 8112593 |
| MeSH | Amino Acid Sequence Base Sequence Cloning, Molecular / methods* DNA Restriction Enzymes / metabolism Escherichia coli / enzymology Escherichia coli / genetics Genetic Vectors* Molecular Sequence Data Plasmids RNA, Messenger / chemistry RNA, Messenger / metabolism Repetitive Sequences, Nucleic Acid* Restriction Mapping* Substrate Specificity beta-Galactosidase / biosynthesis beta-Galactosidase / genetics* beta-Galactosidase / metabolism |
| IF | 2.984 |
| 引用数 | 5 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pTC181 pTC182 pTC191 pTC192 |