RRC ID 33383
Author DeLaFuente A, Cisneros E, Talavera A.
Title Restriction end-converting vectors with tandem repeated multiple cloning sites.
Journal Gene
Abstract The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles.
Volume 139(1)
Pages 83-6
Published 1994-2-11
DOI 10.1016/0378-1119(94)90527-4
PII 0378-1119(94)90527-4
PMID 8112593
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular / methods* DNA Restriction Enzymes / metabolism Escherichia coli / enzymology Escherichia coli / genetics Genetic Vectors* Molecular Sequence Data Plasmids RNA, Messenger / chemistry RNA, Messenger / metabolism Repetitive Sequences, Nucleic Acid* Restriction Mapping* Substrate Specificity beta-Galactosidase / biosynthesis beta-Galactosidase / genetics* beta-Galactosidase / metabolism
IF 2.984
Times Cited 5
Prokaryotes E. coli pTC181 pTC182 pTC191 pTC192