| RRC ID |
33383
|
| 著者 |
DeLaFuente A, Cisneros E, Talavera A.
|
| タイトル |
Restriction end-converting vectors with tandem repeated multiple cloning sites.
|
| ジャーナル |
Gene
|
| Abstract |
The construction, characterization and uses of four vectors with novel multiple cloning sites (MCS) are described. The MCS consist of a central zone with unique restriction sites, flanked by two zones in which four sites are repeated in a direct sense. These vectors allow the insertion of fragments with the same or different restriction ends and their rescue with a multiple combination of ends, identical or different. This system conserves the XGal blue-white selection characteristics of the pUC plasmids, as well as their use as sequencing vehicles.
|
| 巻・号 |
139(1)
|
| ページ |
83-6
|
| 公開日 |
1994-2-11
|
| DOI |
10.1016/0378-1119(94)90527-4
|
| PII |
0378-1119(94)90527-4
|
| PMID |
8112593
|
| MeSH |
Amino Acid Sequence
Base Sequence
Cloning, Molecular / methods*
DNA Restriction Enzymes / metabolism
Escherichia coli / enzymology
Escherichia coli / genetics
Genetic Vectors*
Molecular Sequence Data
Plasmids
RNA, Messenger / chemistry
RNA, Messenger / metabolism
Repetitive Sequences, Nucleic Acid*
Restriction Mapping*
Substrate Specificity
beta-Galactosidase / biosynthesis
beta-Galactosidase / genetics*
beta-Galactosidase / metabolism
|
| IF |
2.984
|
| 引用数 |
5
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 原核生物(大腸菌) |
pTC181
pTC182
pTC191
pTC192 |
