論文 - 詳細
| RRC ID | 33396 |
|---|---|
| 著者 | Nakano Y, Yoshida Y, Yamashita Y, Koga T. |
| タイトル | Construction of a series of pACYC-derived plasmid vectors. |
| ジャーナル | Gene |
| Abstract |
A series of cloning vectors has been constructed from pACYC177 and pACYC184. These vectors contain a MCS, the lacZ alpha reporter gene, and P15A ori. Plasmids pMBL18, pMBL19 and pMBLcos express the bla gene encoding ampicillin resistance, while pMCL200 and pMCL210 contain the cat gene encoding chloramphenicol resistance. In addition, the cosmid pMBLcos was constructed with the P15A ori. These vectors are useful for cloning or subcloning genes which are unstable and/or toxic in high-copy-number vectors. |
| 巻・号 | 162(1) |
| ページ | 157-8 |
| 公開日 | 1995-8-30 |
| DOI | 10.1016/0378-1119(95)00320-6 |
| PII | 0378111995003206 |
| PMID | 7557406 |
| MeSH | Aggregatibacter actinomycetemcomitans / genetics Ampicillin Resistance / genetics Chloramphenicol Resistance / genetics Cloning, Molecular / methods* Genes, Reporter Genetic Vectors* Genomic Library Plasmids / genetics* |
| IF | 2.984 |
| 引用数 | 104 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pMBL18 pMBL19 pMCL200 pMCL210 |