論文 - 詳細
| RRC ID | 33407 |
|---|---|
| 著者 | Kato J, Ikeda H. |
| タイトル | Construction of mini-F plasmid vectors for plasmid shuffling in Escherichia coli. |
| ジャーナル | Gene |
| Abstract |
We have constructed two Escherichia coli mini-F plasmid vectors, pJK286 and pJK289, which have unique EcoRI, BamHI and HindIII sites downstream from the lac promoter. The mini-F vectors are useful for plasmid shuffling, with which we can efficiently carry out localized mutagenesis. |
| 巻・号 | 170(1) |
| ページ | 141-2 |
| 公開日 | 1996-4-17 |
| DOI | 10.1016/0378-1119(95)00865-9 |
| PII | 0378111995008659 |
| PMID | 8621075 |
| MeSH | Chromosomes, Bacterial / genetics Cloning, Molecular / methods* DNA, Bacterial / genetics DNA, Recombinant / genetics Deoxyribonuclease BamHI Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Escherichia coli / genetics* F Factor / genetics* Gene Targeting Genetic Vectors / genetics* Lac Operon / genetics Promoter Regions, Genetic |
| IF | 2.984 |
| 引用数 | 21 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | pJK286 pJK289 |