論文 - 詳細
| RRC ID | 33418 |
|---|---|
| 著者 | Miller WG, Lindow SE. |
| タイトル | An improved GFP cloning cassette designed for prokaryotic transcriptional fusions. |
| ジャーナル | Gene |
| Abstract |
A new gfp cloning cassette designed for prokaryotic transcriptional fusions has been constructed. This cassette consists of gfp (containing the S65T 'red-shift' [Heim et al. (1995) Nature 373, 663-664] and F64L 'protein solubility' [Cormack et al. (1996) Gene 173, 33-38] mutations) flanked by convenient restriction sites, a translational enhancer, and a consensus ribosome binding site with an optimized spacer region. gfp fusion strains containing this cassette demonstrate from 40- to 80-fold greater fluorescence intensity than wild-type gfp fusion strains. Additionally, this cassette confers sufficient fluorescence to recipient cells to be used in low copy-number plasmids, with promoters conferring low levels of transcription, and in bacterial taxa other than Escherichia, such as Pseudomonas. |
| 巻・号 | 191(2) |
| ページ | 149-53 |
| 公開日 | 1997-6-3 |
| DOI | 10.1016/s0378-1119(97)00051-6 |
| PII | S0378111997000516 |
| PMID | 9218713 |
| MeSH | Cloning, Molecular* Escherichia coli / genetics* Fluorescence Gene Expression Genes, Bacterial Genes, Reporter Genetic Vectors* Green Fluorescent Proteins Iron / pharmacology Luminescent Proteins / genetics* Luminescent Proteins / metabolism Mutation Promoter Regions, Genetic Protein Biosynthesis Pseudomonas / genetics* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Solubility Transcription, Genetic |
| IF | 2.984 |
| 引用数 | 183 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pGreen pGreenTIR |