論文 - 詳細
| RRC ID | 33423 |
|---|---|
| 著者 | Remaut E, Tsao H, Fiers W. |
| タイトル | Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication. |
| ジャーナル | Gene |
| Abstract |
Improved expression vectors have been constructed which are derived from runaway-replication mutants of plasmid R1 and carry the strong leftward promoter (pL) of bacteriophage lambda. The activity of this promoter is controlled by a temperature-sensitive repressor, product of the phage gene cI cloned on a compatible plasmid. Heat induction leads to amplification of the plasmid copy number and at the same time turns on the promoter. At a short distance downstream from the promoter, unique EcoRI, BamHI, XbaI and HindIII sites are present. This system was used for high level expression of the T4 DNA-ligase gene; 3 h after induction the ligase amounted to about 20% of total cellular protein. |
| 巻・号 | 22(1) |
| ページ | 103-13 |
| 公開日 | 1983-4-1 |
| DOI | 10.1016/0378-1119(83)90069-0 |
| PII | 0378-1119(83)90069-0 |
| PMID | 6305768 |
| MeSH | Bacteriophage lambda / genetics* Escherichia coli / genetics* Gene Amplification Genetic Vectors* Mutation Operon Plasmids* Replicon Species Specificity Temperature |
| IF | 2.984 |
| 引用数 | 340 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pCP3 pCP40 |