| RRC ID |
33430
|
| 著者 |
Norrander J, Kempe T, Messing J.
|
| タイトル |
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
|
| ジャーナル |
Gene
|
| Abstract |
The restriction endonuclease cleavage sites for SphI and KpnI have been added to the lac cloning region of the phage vectors M13mp10 and M13mp11, using oligodeoxynucleotide-directed in vitro mutagenesis. Complementary deoxy 16-, 21- or 18-mers with the desired base changes were annealed to the M13mp DNA strand and extended with the Klenow fragment of DNA polymerase I. In adding these sites we have shown that this technique can be used as a general method for inserting sequences of DNA as well as introducing deletions and base pair changes.
|
| 巻・号 |
26(1)
|
| ページ |
101-6
|
| 公開日 |
1983-12-1
|
| DOI |
10.1016/0378-1119(83)90040-9
|
| PII |
0378-1119(83)90040-9
|
| PMID |
6323249
|
| MeSH |
Base Sequence
Cloning, Molecular
Coliphages / genetics*
DNA Restriction Enzymes
Genetic Vectors*
Mutation*
Oligodeoxyribonucleotides*
Oligonucleotides*
|
| IF |
2.984
|
| 引用数 |
2319
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 原核生物(大腸菌) |
M13mp18
M13mp19 |
