Reference - Detail
| RRC ID | 33467 |
|---|---|
| Author | Kretschmer FJ, Chang AC, Cohen SN. |
| Title | Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. |
| Journal | J Bacteriol |
| Abstract |
A procedure is described that uses an indicator plasmid (pSC201) to identify cells in a bacterial population that have been co-transformed with a second plasmid lacking detectable phenotypic properties. Under appropriate conditions of indirect selection, between 50 and 85% of transformants carrying the indicator plasmid also contain the nonselected plasmid. A temperature-sensitive mutation in the replication functions of the indicator plasmid enables its elimination from doubly transformed bacteria. Using this procedure, we have isolated bacteria that carry only the small cryptic plasmid. P15A, of the Escherichia coli strain 15. This genetic element, which contains only 2,300 nucleotide pairs, is thus capable of functioning as a replicon independently of the two larger plasmids normally associated with it in E. coli 15 strains (Ikeda, Inuzuka, and Tomizawa, 1970). |
| Volume | 124(1) |
| Pages | 225-31 |
| Published | 1975-10-1 |
| DOI | 10.1128/jb.124.1.225-231.1975 |
| PMID | 1100600 |
| PMC | PMC235886 |
| MeSH | DNA Replication DNA, Bacterial* / analysis DNA, Bacterial* / biosynthesis Escherichia coli* / analysis Escherichia coli* / metabolism Extrachromosomal Inheritance* Methylnitronitrosoguanidine Molecular Weight Mutagens Mutation Phenotype Plasmids* Temperature Transformation, Genetic |
| IF | 3.006 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pSC201 |