| RRC ID |
33482
|
| 著者 |
Armstrong KA, Acosta R, Ledner E, Machida Y, Pancotto M, McCormick M, Ohtsubo H, Ohtsubo E.
|
| タイトル |
A 37 X 10(3) molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1.
|
| ジャーナル |
J Mol Biol
|
| Abstract |
Nucleotide sequences were determined for a region essential for autonomous replication and partitioning of pSC101, a plasmid whose replication is dependent on the Escherichia coli dnaA gene product. The essential replication region contains one long coding sequence, rep101 , for a protein composed of 316 amino acids, and a polypeptide approximately 37 X 10(3) Mr in size was identified as the rep101 gene product. rep101 is preceded by two inverted repeat sequences, three directly repeated sequences and a region of high A + T content containing a sequence similar to the E. coli oriC consensus sequence. Because the lesions in seven replication-deficient insertion mutants, four mutants with increased copy number and one temperature-sensitive replication mutant occur within rep101 , the rep101 gene product must control pSC101 replication and copy number. par, a region adjacent to the replication region, which functions in stable plasmid inheritance, contains several inverted repeat sequences.
|
| 巻・号 |
175(3)
|
| ページ |
331-48
|
| 公開日 |
1984-5-25
|
| DOI |
10.1016/0022-2836(84)90352-8
|
| PII |
0022-2836(84)90352-8
|
| PMID |
6327996
|
| MeSH |
Autoradiography
Bacterial Proteins*
Base Sequence
Chromosome Mapping
DNA Replication*
Escherichia coli / genetics*
Molecular Weight
Mutation
Plasmids*
Repetitive Sequences, Nucleic Acid
Temperature
|
| IF |
4.76
|
| 引用数 |
118
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
pEL3 |
