論文 - 詳細
| RRC ID | 33528 |
|---|---|
| 著者 | Umemiya T, Takeichi M, Nose A. |
| タイトル | M-spondin, a novel ECM protein highly homologous to vertebrate F-spondin, is localized at the muscle attachment sites in the Drosophila embryo. |
| ジャーナル | Dev Biol |
| Abstract |
The muscle attachment site (MAS) in Drosophila provides a unique and excellent model system to study the mechanism of cell-matrix adhesion in developing organisms. Here, we report on the isolation and characterization of a novel extracellular matrix (ECM) molecule localized at the MAS, encoded by the M-spondin (mspo) gene. M-spondin protein contains a thrombospondin type I repeat (TSR) previously found in a variety of ECM molecules. Furthermore, it shares two conserved domains with F-spondin, a vertebrate ECM molecule with TSRs. The presence of TSR(s) and the two homologous domains thus defines a novel gene family of ECM molecules. The mspo mRNA was expressed by a large subset of muscles in the embryonic body wall. Secreted M-spondin protein diffused and eventually became immobilized at the MAS in late embryos. When expressed in S2 cells, the protein was secreted and became concentrated in the matrix on the surface of the culture dish. Genetic analysis revealed that both deletion mutants and misexpression mutants suffered no obvious developmental defects. We propose that M-spondin, although its function is redundant, is a component of the ECM and mediates mechanical linkage between the muscles and apodemes. |
| 巻・号 | 186(2) |
| ページ | 165-76 |
| 公開日 | 1997-6-15 |
| DOI | 10.1006/dbio.1997.8591 |
| PII | S0012-1606(97)98591-2 |
| PMID | 9205137 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cells, Cultured DNA, Complementary / chemistry DNA, Complementary / isolation & purification Drosophila / embryology* Drosophila Proteins Extracellular Matrix Proteins / analysis* Extracellular Matrix Proteins / chemistry Extracellular Matrix Proteins / genetics Gene Expression Growth Substances* Membrane Proteins Microscopy, Electron Molecular Sequence Data Muscles / chemistry Muscles / embryology* Neural Cell Adhesion Molecules / chemistry* Neural Cell Adhesion Molecules / genetics Peptides* RNA, Messenger / metabolism Sequence Analysis Sequence Homology* |
| IF | 2.896 |
| 引用数 | 51 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
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