| RRC ID |
33548
|
| Author |
Fukushima T, Tanabe T, Yamamoto H, Hosoya S, Sato T, Yoshikawa H, Sekiguchi J.
|
| Title |
Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis.
|
| Journal |
J Biochem
|
| Abstract |
The predicted amino acid sequence of Bacillus subtilis ybaN (renamed pdaB) exhibits high similarity to those of several polysaccharide deacetylases. Northern hybridization analysis with sporulation sigma mutants indicated that the pdaB gene is transcribed by EsigmaE RNA polymerase and negatively regulated by SpoIIID. The pdaB mutant was deficient in spore formation. Phase- and electron microscopic observation showed morphological changes of spores in late sporulation periods. The pdaB spores that had lost their viability were empty. Moreover, GFP driven by the promoter of the sspE gene was localized in the forespore compartment for the wild type, but was localized in both the mother cell and forespore compartments for phase-gray/dark forespores of the pdaB mutant. This indicates that GFP expressed in the forespores of the mutant leaks into the mother cells. Therefore, PdaB is necessary to maintain spores after the late stage of sporulation.
|
| Volume |
136(3)
|
| Pages |
283-91
|
| Published |
2004-9-1
|
| DOI |
10.1093/jb/mvh151
|
| PII |
136/3/283
|
| PMID |
15598884
|
| MeSH |
Amidohydrolases / genetics*
Amidohydrolases / metabolism
Bacillus subtilis / genetics*
Bacillus subtilis / metabolism*
Bacterial Proteins / chemistry
Base Sequence
Blotting, Northern
Cell Survival
Centrifugation, Density Gradient
DNA Primers / chemistry
DNA-Binding Proteins / chemistry
DNA-Directed RNA Polymerases / chemistry
Escherichia coli / metabolism
Genetic Complementation Test
Genotype
Green Fluorescent Proteins / metabolism
Microscopy, Electron
Microscopy, Phase-Contrast
Molecular Sequence Data
Mutation
Picolinic Acids / chemistry
Plasmids / metabolism
Polysaccharides / chemistry
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Spores, Bacterial / metabolism*
Time Factors
Transcription Factors / chemistry
|
| IF |
2.476
|
| Times Cited |
18
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes B. subtilis |
MBS56
MBS57
MBS58 |
