| RRC ID |
33615
|
| Author |
Almeida AD, Boije H, Chow RW, He J, Tham J, Suzuki SC, Harris WA.
|
| Title |
Spectrum of Fates: a new approach to the study of the developing zebrafish retina.
|
| Journal |
Development
|
| Abstract |
The ability to image cells live and in situ as they proliferate and differentiate has proved to be an invaluable asset to biologists investigating developmental processes. Here, we describe a Spectrum of Fates approach that allows the identification of all the major neuronal subtypes in the zebrafish retina simultaneously. Spectrum of Fates is based on the combinatorial expression of differently coloured fluorescent proteins driven by the promoters of transcription factors that are expressed in overlapping subsets of retinal neurons. Here, we show how a Spectrum of Fates approach can be used to assess various aspects of neural development, such as developmental waves of differentiation, neuropil development, lineage tracing and hierarchies of fates in the developing zebrafish retina.
|
| Volume |
141(9)
|
| Pages |
1971-80
|
| Published |
2014-5-1
|
| DOI |
10.1242/dev.104760
|
| PII |
dev.104760
|
| PMID |
24718991
|
| PMC |
PMC3994774
|
| MeSH |
Animals
Animals, Genetically Modified
Cell Differentiation
Cell Lineage
Cell Proliferation
Cells, Cultured
Clone Cells
Fluorescence
Genetic Techniques*
Luminescent Proteins / metabolism*
Retina / cytology
Retina / embryology*
Zebrafish / embryology*
Zebrafish / genetics*
|
| IF |
5.611
|
| Times Cited |
20
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Zebrafish |
Tg(chx10:GFP)
Tg(vsx2:EGFP) |
