RRC ID 33622
Author Miyasaka N, Arganda-Carreras I, Wakisaka N, Masuda M, Sümbül U, Seung HS, Yoshihara Y.
Title Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling.
Journal Nat Commun
Abstract Chemotopic odour representations in the olfactory bulb are transferred to multiple forebrain areas and translated into appropriate output responses. However, a comprehensive projection map of bulbar output neurons at single-axon resolution is lacking in vertebrates. Here we unravel a projectome of the zebrafish olfactory bulb through genetic single-neuron tracing and image registration. We show that five major target regions receive distinct modes of projections from olfactory bulb glomeruli. The central portion of posterior telencephalon receives non-selective, interspersed inputs from all glomeruli, whereas the ventral telencephalon is diffusely innervated by axons from particular glomerular clusters. The right habenula and posterior tuberculum (diencephalic nuclei) receive convergent inputs from restricted and all glomerular clusters, respectively. The bulbar recurrent projections are coarsely topographic. Thus, the primary chemotopic organization is transformed into distinct sensory representations in higher olfactory centres. These findings provide a framework to understand general principles as well as species-specific features in decoding of odour information.
Volume 5
Pages 3639
Published 2014-4-9
DOI 10.1038/ncomms4639
PII ncomms4639
PMID 24714622
MeSH Animals Axons / metabolism Olfactory Bulb / metabolism* Prosencephalon / metabolism Telencephalon / metabolism Zebrafish
IF 11.878
Times Cited 11
WOS Category NEUROSCIENCES
Resource
Zebrafish rw0310(Tg(lhx2a:GFP)) rw0311( Tg(lhx2a:gap-YFP))