論文 - 詳細
| RRC ID | 33690 |
|---|---|
| 著者 | Li X, Roszko I, Sepich DS, Ni M, Hamm HE, Marlow FL, Solnica-Krezel L. |
| タイトル | Gpr125 modulates Dishevelled distribution and planar cell polarity signaling. |
| ジャーナル | Development |
| Abstract |
During vertebrate gastrulation, Wnt/planar cell polarity (PCP) signaling orchestrates polarized cell behaviors underlying convergence and extension (C&E) movements to narrow embryonic tissues mediolaterally and lengthen them anteroposteriorly. Here, we have identified Gpr125, an adhesion G protein-coupled receptor, as a novel modulator of the Wnt/PCP signaling system. Excess Gpr125 impaired C&E movements and the underlying cell and molecular polarities. Reduced Gpr125 function exacerbated the C&E and facial branchiomotor neuron (FBMN) migration defects of embryos with reduced Wnt/PCP signaling. At the molecular level, Gpr125 recruited Dishevelled to the cell membrane, a prerequisite for Wnt/PCP activation. Moreover, Gpr125 and Dvl mutually clustered one another to form discrete membrane subdomains, and the Gpr125 intracellular domain directly interacted with Dvl in pull-down assays. Intriguingly, Dvl and Gpr125 were able to recruit a subset of PCP components into membrane subdomains, suggesting that Gpr125 may modulate the composition of Wnt/PCP membrane complexes. Our study reveals a role for Gpr125 in PCP-mediated processes and provides mechanistic insight into Wnt/PCP signaling. |
| 巻・号 | 140(14) |
| ページ | 3028-39 |
| 公開日 | 2013-7-1 |
| DOI | 10.1242/dev.094839 |
| PII | 140/14/3028 |
| PMID | 23821037 |
| PMC | PMC3699285 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Animals Cell Movement* Cell Polarity* Dishevelled Proteins Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Mutation Phosphoproteins / metabolism Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism* Wings, Animal / cytology Wings, Animal / embryology Wnt Signaling Pathway* Zebrafish / embryology* Zebrafish / metabolism* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 5.611 |
| 引用数 | 35 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(isl1:GFP)/rw0 |