論文 - 詳細
| RRC ID | 3389 |
|---|---|
| 著者 | Kadandale P, Stewart-Michaelis A, Gordon S, Rubin J, Klancer R, Schweinsberg P, Grant BD, Singson A. |
| タイトル | The egg surface LDL receptor repeat-containing proteins EGG-1 and EGG-2 are required for fertilization in Caenorhabditis elegans. |
| ジャーナル | Curr Biol |
| Abstract |
The molecular machinery that mediates sperm-egg interactions at fertilization is largely unknown. We identify two partially redundant egg surface LDL receptor repeat-containing proteins (EGG-1 and EGG-2) that are required for Caenorhabditis elegans fertility in hermaphrodites, but not males. Wild-type sperm cannot enter the morphologically normal oocytes produced by hermaphrodites that lack egg-1 and egg-2 function despite direct gamete contact. Furthermore, we find that levels of meiotic maturation/ovulation and sperm migratory behavior are altered in egg-1 mutants. These observations suggest an unexpected regulatory link between fertilization and other events necessary for reproductive success. egg-1 and egg-2 are the result of a gene duplication in the nematode lineage leading to C. elegans. The two closely related species C. briggsae and C. remanei encode only a single egg-1/egg-2 homolog that is required for hermaphrodite/female fertility. In addition to being the first identified egg components of the nematode fertilization machinery, the egg-1 and egg-2 gene duplication could be vital with regards to maximizing C. elegans fecundity and understanding the evolutionary differentiation of molecular function and speciation. |
| 巻・号 | 15(24) |
| ページ | 2222-9 |
| 公開日 | 2005-12-20 |
| DOI | 10.1016/j.cub.2005.10.043 |
| PII | S0960-9822(05)01296-0 |
| PMID | 16360684 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cluster Analysis Computational Biology Female Fertilization / physiology* Green Fluorescent Proteins Male Ovum / metabolism* Phylogeny* RNA Interference Receptors, Cell Surface / metabolism* Receptors, LDL / metabolism* Sequence Analysis, DNA Sperm-Ovum Interactions / physiology* |
| IF | 9.601 |
| 引用数 | 35 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1071 |