RRC ID 339
Author Takehana A, Yano T, Mita S, Kotani A, Oshima Y, Kurata S.
Title Peptidoglycan recognition protein (PGRP)-LE and PGRP-LC act synergistically in Drosophila immunity.
Journal EMBO J.
Abstract In innate immunity, pattern recognition molecules recognize cell wall components of microorganisms and activate subsequent immune responses, such as the induction of antimicrobial peptides and melanization in Drosophila. The diaminopimelic acid (DAP)-type peptidoglycan potently activates imd-dependent induction of antibacterial peptides. Peptidoglycan recognition protein (PGRP) family members act as pattern recognition molecules. PGRP-LC loss-of-function mutations affect the imd-dependent induction of antibacterial peptides and resistance to Gram-negative bacteria, whereas PGRP-LE binds to the DAP-type peptidoglycan, and a gain-of-function mutation induces constitutive activation of both the imd pathway and melanization. Here, we generated PGRP-LE null mutants and report that PGRP-LE functions synergistically with PGRP-LC in producing resistance to Escherichia coli and Bacillus megaterium infections, which have the DAP-type peptidoglycan. Consistent with this, PGRP-LE acts both upstream and in parallel with PGRP-LC in the imd pathway, and is required for infection-dependent activation of melanization in Drosophila. A role for PGRP-LE in the epithelial induction of antimicrobial peptides is also suggested.
Volume 23(23)
Pages 4690-700
Published 2004-11-24
DOI 10.1038/sj.emboj.7600466
PII 7600466
PMID 15538387
PMC PMC533052
MeSH Animals Bacillus megaterium Carrier Proteins / genetics Carrier Proteins / immunology* Drosophila / immunology* Drosophila / microbiology* Drosophila Proteins / biosynthesis Drosophila Proteins / genetics Drosophila Proteins / immunology* Epithelium / immunology Epithelium / metabolism Escherichia coli Gene Expression Regulation Mutation Peptides / genetics Peptides / immunology*
IF 11.227
Times Cited 165