RRC ID 3391
Author Nakamura K, Kim S, Ishidate T, Bei Y, Pang K, Shirayama M, Trzepacz C, Brownell DR, Mello CC.
Title Wnt signaling drives WRM-1/beta-catenin asymmetries in early C. elegans embryos.
Journal Genes Dev.
Abstract beta-Catenin regulates cell adhesion and cellular differentiation during development, and misregulation of beta-catenin contributes to numerous forms of cancer in humans. Here we describe Caenorhabditis elegans conditional alleles of mom-2/Wnt, mom-4/Tak1, and wrm-1/beta-catenin. We use these reagents to examine the regulation of WRM-1/beta-catenin during a Wnt-signaling-induced asymmetric cell division. While WRM-1 protein initially accumulates in the nuclei of all cells, signaling promotes the retention of WRM-1 in nuclei of responding cells. We show that both PRY-1/Axin and the nuclear exportin homolog IMB-4/CRM-1 antagonize signaling. These findings reveal how Wnt signals direct the asymmetric localization of beta-catenin during polarized cell division.
Volume 19(15)
Pages 1749-54
Published 2005-8-1
DOI 10.1101/gad.1323705
PII 19/15/1749
PMID 16077004
PMC PMC1182335
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Cell Division Cell Nucleus / metabolism Cell Polarity Cytoskeletal Proteins / metabolism* Green Fluorescent Proteins / metabolism Intercellular Signaling Peptides and Proteins / metabolism* Membrane Proteins / chemistry Membrane Proteins / metabolism Molecular Sequence Data Sequence Homology, Amino Acid Signal Transduction* Trans-Activators / metabolism* Wnt Proteins beta Catenin
IF 8.99
Times Cited 58
C.elegans tm514