Reference - Detail
| RRC ID | 3407 |
|---|---|
| Author | Bae YK, Qin H, Knobel KM, Hu J, Rosenbaum JL, Barr MM. |
| Title | General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia. |
| Journal | Development |
| Abstract |
Ciliary localization of the transient receptor potential polycystin 2 channel (TRPP2/PKD-2) is evolutionarily conserved, but how TRPP2 is targeted to cilia is not known. In this study, we characterize the motility and localization of PKD-2, a TRPP2 homolog, in C. elegans sensory neurons. We demonstrate that GFP-tagged PKD-2 moves bidirectionally in the dendritic compartment. Furthermore, we show a requirement for different molecules in regulating the ciliary localization of PKD-2. PKD-2 is directed to moving dendritic particles by the UNC-101/adaptor protein 1 (AP-1) complex. When expressed in non-native neurons, PKD-2 remains in cell bodies and is not observed in dendrites or cilia, indicating that cell-type specific factors are required for directing PKD-2 to the dendrite. PKD-2 stabilization in cilia and cell bodies requires LOV-1, a functional partner and a TRPP1 homolog. In lov-1 mutants, PKD-2 is greatly reduced in cilia and forms abnormal aggregates in neuronal cell bodies. Intraflagellar transport (IFT) is not essential for PKD-2 dendritic motility or access to the cilium, but may regulate PKD-2 ciliary abundance. We propose that both general and cell-type-specific factors govern TRPP2/PKD-2 subcellular distribution by forming at least two steps involving somatodendritic and ciliary sorting decisions. |
| Volume | 133(19) |
| Pages | 3859-70 |
| Published | 2006-10-1 |
| DOI | 10.1242/dev.02555 |
| PII | dev.02555 |
| PMID | 16943275 |
| MeSH | Adaptor Protein Complex 1 / metabolism Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / chemistry Cilia / metabolism Cilia / ultrastructure Dendrites / chemistry Dendrites / metabolism* Dendrites / ultrastructure Endoplasmic Reticulum / chemistry Endoplasmic Reticulum / metabolism Female Flagella / chemistry Flagella / metabolism Green Fluorescent Proteins / analysis Male Neurons, Afferent / chemistry Neurons, Afferent / metabolism* Neurons, Afferent / ultrastructure Protein Transport TRPP Cation Channels / analysis TRPP Cation Channels / metabolism* |
| IF | 5.611 |
| Times Cited | 70 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm324 tm920 tm375 tm394 |