RRC ID 34103
Author Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T.
Title Characterization of a novel β-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 protein family member.
Journal J Biol Chem
Abstract Pfam DUF1680 (PF07944) is an uncharacterized protein family conserved in many species of bacteria, actinomycetes, fungi, and plants. Previously, we cloned and characterized the hypBA2 gene as a β-L-arabinobiosidase in Bifidobacterium longum JCM 1217. In this study, we cloned a DUF1680 family member, the hypBA1 gene, which constitutes a gene cluster with hypBA2. HypBA1 is a novel β-L-arabinofuranosidase that liberates L-arabinose from the L-arabinofuranose (Araf)-β1,2-Araf disaccharide. HypBA1 also transglycosylates 1-alkanols with retention of the anomeric configuration. Mutagenesis and azide rescue experiments indicated that Glu-338 is a critical residue for catalytic activity. This study provides the first characterization of a DUF1680 family member, which defines a new family of glycoside hydrolases, the glycoside hydrolase family 127.
Volume 289(8)
Pages 5240-9
Published 2014-2-21
DOI 10.1074/jbc.M113.528711
PII M113.528711
PMID 24385433
PMC PMC3931080
MeSH Amino Acids / metabolism Arabinose / analogs & derivatives Arabinose / chemistry Arabinose / metabolism Bacterial Proteins / chemistry* Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Bifidobacterium / enzymology* Bifidobacterium / growth & development Chromatography, High Pressure Liquid Chromatography, Ion Exchange Chromatography, Thin Layer Electrophoresis, Polyacrylamide Gel Fermentation Glycoproteins / metabolism Glycoside Hydrolases / chemistry Glycoside Hydrolases / metabolism* Glycosylation Hydrogen-Ion Concentration Kinetics Magnetic Resonance Spectroscopy Models, Biological Molecular Sequence Data Mutagenesis, Site-Directed Mutant Proteins / metabolism Recombinant Proteins / metabolism Sequence Analysis, Protein Substrate Specificity Temperature
IF 4.238
Times Cited 22
General Microbes JCM 1192 JCM 1205 JCM 1217 JCM 1222 JCM 1254 JCM 1275 JCM 7054