RRC ID 34103
Author Fujita K, Takashi Y, Obuchi E, Kitahara K, Suganuma T.
Title Characterization of a novel β-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 protein family member.
Journal J. Biol. Chem.
Abstract Pfam DUF1680 (PF07944) is an uncharacterized protein family conserved in many species of bacteria, actinomycetes, fungi, and plants. Previously, we cloned and characterized the hypBA2 gene as a β-L-arabinobiosidase in Bifidobacterium longum JCM 1217. In this study, we cloned a DUF1680 family member, the hypBA1 gene, which constitutes a gene cluster with hypBA2. HypBA1 is a novel β-L-arabinofuranosidase that liberates L-arabinose from the L-arabinofuranose (Araf)-β1,2-Araf disaccharide. HypBA1 also transglycosylates 1-alkanols with retention of the anomeric configuration. Mutagenesis and azide rescue experiments indicated that Glu-338 is a critical residue for catalytic activity. This study provides the first characterization of a DUF1680 family member, which defines a new family of glycoside hydrolases, the glycoside hydrolase family 127.
Volume 289(8)
Pages 5240-9
Published 2014-2-21
DOI 10.1074/jbc.M113.528711
PII M113.528711
PMID 24385433
PMC PMC3931080
MeSH Amino Acids / metabolism Arabinose / analogs & derivatives Arabinose / chemistry Arabinose / metabolism Bacterial Proteins / chemistry* Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Bifidobacterium / enzymology* Bifidobacterium / growth & development Chromatography, High Pressure Liquid Chromatography, Ion Exchange Chromatography, Thin Layer Electrophoresis, Polyacrylamide Gel Fermentation Glycoproteins / metabolism Glycoside Hydrolases / chemistry Glycoside Hydrolases / metabolism* Glycosylation Hydrogen-Ion Concentration Kinetics Magnetic Resonance Spectroscopy Models, Biological Molecular Sequence Data Mutagenesis, Site-Directed Mutant Proteins / metabolism Recombinant Proteins / metabolism Sequence Analysis, Protein Substrate Specificity Temperature
IF 4.106
Times Cited 9
General Microbes JCM 1192 JCM 1205 JCM 1217 JCM 1222 JCM 1254 JCM 1275 JCM 7054