論文 - 詳細
| RRC ID | 3415 |
|---|---|
| 著者 | Carroll BT, Dubyak GR, Sedensky MM, Morgan PG. |
| タイトル | Sulfated signal from ASJ sensory neurons modulates stomatin-dependent coordination in Caenorhabditis elegans. |
| ジャーナル | J Biol Chem |
| Abstract |
The neuronal stomatin-like proteins UNC-1 and UNC-24 play important roles in the nervous system of Caenorhabditis elegans. These neuronal stomatin-like proteins are putative chaperone proteins that can modify volatile anesthetic sensitivity and disrupt coordinated locomotion. A suppressor of unc-1 and unc-24, named ssu-1(fc73) (for suppressor of stomatin uncoordination), suppresses three phenotypes of neuronal stomatin-like protein deficiency as follows: volatile anesthetic sensitivity, uncoordinated locomotion, and a constitutive alternative developmental phenotype known as dauer. Here we provide the first phenotypic characterization of ssu-1, predicted to be the only C. elegans cytosolic alcohol sulfotransferase, a family of enzymes that catalyze a sulfate linkage with the alcohol group of small molecules for the purposes of detoxification or modification of signaling. In vitro enzyme analysis of bacterially expressed SSU-1 demonstrates sulfotransferase activity and thus confirms the function predicted by protein sequence similarities. Whereas unc-1 is expressed in the majority of neurons of C. elegans, expression of SSU-1 protein in only the two ASJ amphid interneurons is sufficient to restore the wild type phenotype. This work demonstrates that SSU-1 is a functional sulfotransferase that likely modifies endocrine signaling in C. elegans. The expression of SSU-1 in the ASJ neurons refines the understanding of the function of these cells and supports their classification as endocrine tissue. The relationship of unc-1, unc-24, and ssu-1 is the first association of neuronal stomatin-like proteins sharing regulatory roles with a sulfotransferase enzyme. |
| 巻・号 | 281(47) |
| ページ | 35989-96 |
| 公開日 | 2006-11-24 |
| DOI | 10.1074/jbc.M606086200 |
| PII | S0021-9258(20)68149-2 |
| PMID | 16973616 |
| MeSH | Alleles Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism Cytosol / metabolism Gene Expression Regulation* Membrane Proteins / chemistry Membrane Proteins / metabolism Models, Biological Models, Genetic Neurons, Afferent / metabolism* Protein Isoforms Signal Transduction Sulfates / metabolism Sulfotransferases / chemistry Sulfotransferases / metabolism |
| IF | 4.238 |
| 引用数 | 15 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1117 |