| RRC ID |
3421
|
| Author |
Coustham V, Bedet C, Monier K, Schott S, Karali M, Palladino F.
|
| Title |
The C. elegans HP1 homologue HPL-2 and the LIN-13 zinc finger protein form a complex implicated in vulval development.
|
| Journal |
Dev Biol
|
| Abstract |
HP1 proteins are essential components of heterochromatin and contribute to the transcriptional repression of euchromatic genes via the recruitment to specific promoters by corepressor proteins including TIF1 and Rb. The Caenorhabditis elegans HP1 homologue HPL-2 acts in the "synMuv" (synthetic multivulval) pathway, which defines redundant negative regulators of a Ras signaling cascade required for vulval induction. Several synMuv genes encode for chromatin-associated proteins involved in transcriptional regulation, including Rb and components of the Mi-2/NuRD and TIP60/NuA4 chromatin remodeling complexes. Here, we show that HPL-2 physically interacts in vitro and in vivo with the multiple zinc finger protein LIN-13, another member of the synMuv pathway. A variant of the conserved PXVXL motif found in many HP1-interacting proteins mediates LIN-13 binding to the CSD of HPL-2. We further show by in vivo localization studies that LIN-13 is required for HPL-2 recruitment in nuclear foci. Our data suggest that the LIN-13/HPL-2 complex may physically link a subset of the Rb related synMuv proteins to chromatin.
|
| Volume |
297(2)
|
| Pages |
308-22
|
| Published |
2006-9-15
|
| DOI |
10.1016/j.ydbio.2006.04.474
|
| PII |
S0012-1606(06)00728-7
|
| PMID |
16890929
|
| MeSH |
Amino Acid Motifs
Amino Acid Sequence
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / physiology*
Carrier Proteins / chemistry
Carrier Proteins / physiology*
Chromobox Protein Homolog 5
Chromosomal Proteins, Non-Histone / chemistry
Chromosomal Proteins, Non-Histone / physiology*
Conserved Sequence
Female
Gene Expression Regulation, Developmental*
Models, Genetic
Molecular Sequence Data
Protein Binding
Sequence Homology, Amino Acid
Vulva / embryology*
|
| IF |
2.896
|
| Times Cited |
34
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| C.elegans |
tm1489 |
