| RRC ID |
34338
|
| 著者 |
Choi JH, Lee KM, Lee MK, Cha CJ, Kim GB.
|
| タイトル |
Bifidobacterium faecale sp. nov., isolated from human faeces.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel strain, designated strain CU3-7(T), was isolated from faeces of a two-week-old baby. The isolate was Gram-staining-positive, anaerobic and rod-shaped. Results from 16S rRNA gene sequence analysis revealed that strain CU3-7(T) was phylogenetically affiliated with members of the genus Bifidobacterium. Strain CU3-7(T) showed the highest level of sequence similarity with Bifidobacterium adolescentis KCTC 3216(T) (98.4 %), followed by Bifidobacterium ruminantium KCTC 3425(T) (97.9 %). Analysis of hsp60 sequences showed that strain CU3-7(T) was closely related to B. adolescentis KCTC 3216(T) (94.0 %) and B. ruminantium KCTC 3425(T) (92.5 %). The DNA-DNA hybridization values with the closely related strains were all below the cut-off value for species delineation, 17.0 % with B. ruminantium KCTC 3425(T) and 14.9 % with B. adolescentis KCTC 3216(T). Fructose-6-phosphate phosphoketolase activity was detected. The predominant cellular fatty acids were C16 : 0 (27.7 %), C18 : 1ω9c (27.4 %) and C18 : 1ω9c dimethylacetate (15.5 %). The DNA G+C content was 58.6 mol%. On the basis of polyphasic taxonomy, strain CU3-7(T) should be classified as the type strain of a novel species within the genus Bifidobacterium, for which the name Bifidobacterium faecale sp. nov. is proposed ( = KACC 17904(T) = JCM 19861(T)).
|
| 巻・号 |
64(Pt 9)
|
| ページ |
3134-3139
|
| 公開日 |
2014-9-1
|
| DOI |
10.1099/ijs.0.063479-0
|
| PMID |
24944342
|
| MeSH |
Aldehyde-Lyases / metabolism
Bacterial Typing Techniques
Base Composition
Bifidobacterium / classification*
Bifidobacterium / genetics
Bifidobacterium / isolation & purification
Chaperonin 60 / genetics
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology*
Humans
Infant, Newborn
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.415
|
| 引用数 |
8
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 19861 |
