| RRC ID |
34346
|
| 著者 |
Mino S, Kudo H, Arai T, Sawabe T, Takai K, Nakagawa S.
|
| タイトル |
Sulfurovum aggregans sp. nov., a hydrogen-oxidizing, thiosulfate-reducing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent chimney, and an emended description of the genus Sulfurovum.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel mesophilic, strictly hydrogen-oxidizing, sulfur-, nitrate- and thiosulfate-reducing bacterium, designated strain Monchim33(T), was isolated from a deep-sea hydrothermal vent chimney at the Central Indian Ridge. The non-motile, rod-shaped cells were Gram-stain-negative and non-sporulating. Growth was observed between 15 and 37 °C (optimum 33 °C; 3.2 h doubling time) and between pH 5.4 and 8.6 (optimum pH 6.0). The isolate was a strictly anaerobic chemolithoautotroph capable of using molecular hydrogen as the sole energy source and carbon dioxide as the sole carbon source. The G+C content of the genomic DNA was 42.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate belonged to the genus Sulfurovum and was closely related to Sulfurovum sp. NBC37-1 and Sulfurovum lithotrophicum 42BK(T) (95.6 and 95.4 % similarity, respectively). DNA-DNA hybridization demonstrated that the novel isolate could be differentiated genotypically from Sulfurovum sp. NBC37-1 and Sulfurovum lithotrophicum. On the basis of the molecular and physiological traits of the new isolate, the name Sulfurovum aggregans sp. nov. is proposed, with the type strain Monchim33(T) ( = JCM 19824(T) = DSM 27205(T)).
|
| 巻・号 |
64(Pt 9)
|
| ページ |
3195-3201
|
| 公開日 |
2014-9-1
|
| DOI |
10.1099/ijs.0.065094-0
|
| PMID |
24966202
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Chemoautotrophic Growth
DNA, Bacterial / genetics
Epsilonproteobacteria / classification*
Epsilonproteobacteria / genetics
Epsilonproteobacteria / isolation & purification
Fatty Acids / chemistry
Hydrogen / metabolism
Hydrothermal Vents / microbiology*
Molecular Sequence Data
Nucleic Acid Hybridization
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seawater / microbiology
Sequence Analysis, DNA
Thiosulfates / metabolism
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
| IF |
2.415
|
| 引用数 |
39
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 19824 |
