RRC ID 34370
Author Konishi M, Maruoka N, Furuta Y, Morita T, Fukuoka T, Imura T, Kitamoto D.
Title Biosurfactant-producing yeasts widely inhabit various vegetables and fruits.
Journal Biosci Biotechnol Biochem
Abstract The isolation of biosurfactant-producing yeasts from food materials was accomplished. By a combination of a new drop collapse method and thin-layer chromatography, 48 strains were selected as glycolipid biosurfactant producers from 347 strains, which were randomly isolated from various vegetables and fruits. Of the producers, 69% were obtained from vegetables of the Brassica family. Of the 48 producers, 15 strains gave relatively high yields of mannosylerythritol lipids (MELs), and were identified as Pseudozyma yeasts. These strains produced MELs from olive oil at yields ranging from 8.5 to 24.3 g/L. The best yield coefficient reached 0.49 g/g as to the carbon sources added. Accordingly, MEL producers were isolated at high efficiency from various vegetables and fruits, indicating that biosurfactant producers are widely present in foods. The present results should facilitate their application in the food and related industries.
Volume 78(3)
Pages 516-23
Published 2014-1-1
DOI 10.1080/09168451.2014.882754
PMID 25036844
MeSH Fruit / microbiology Glycolipids / chemistry Glycolipids / isolation & purification* Olive Oil Plant Oils / chemistry Surface-Active Agents / chemistry Surface-Active Agents / isolation & purification* Vegetables / microbiology Yeasts / chemistry Yeasts / isolation & purification*
IF 1.516
Times Cited 5
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 10324