論文 - 詳細
| RRC ID | 34430 |
|---|---|
| 著者 | Sánchez-Andrea I, Sanz JL, Stams AJM. |
| タイトル | Microbacter margulisiae gen. nov., sp. nov., a propionigenic bacterium isolated from sediments of an acid rock drainage pond. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel anaerobic propionigenic bacterium, strain ADRI(T), was isolated from sediment of an acid rock drainage environment (Tinto River, Spain). Cells were small (0.4-0.6×1-1.7 µm), non-motile and non-spore-forming rods. Cells possessed a Gram-negative cell-wall structure and were vancomycin-resistant. Strain ADRI(T) utilized yeast extract and various sugars as substrates and formed propionate, lactate and acetate as major fermentation products. The optimum growth temperature was 30 °C and the optimum pH for growth was pH 6.5, but strain ADRI(T) was able to grow at a pH as low as 3.0. Oxidase, indole formation, and urease and catalase activities were negative. Aesculin and gelatin were hydrolysed. The predominant cellular fatty acids of strain ADRI(T) were anteiso-C15 : 0 (30.3 %), iso-C15 : 0 (29.2 %) and iso-C17 : 0 3-OH (14.9 %). Major menaquinones were MK-8 (52 %) and MK-9 (48 %). The genomic DNA G+C content was 39.9 mol%. Phylogenetically, strain ADRI(T) was affiliated to the family Porphyromonadaceae of the phylum Bacteroidetes. The most closely related cultured species were Paludibacter propionicigenes with 16S rRNA gene sequence similarity of 87.5 % and several species of the genus Dysgonomonas (similarities of 83.5-85.4 % to the type strains). Based on the distinctive ecological, phenotypic and phylogenetic characteristics of strain ADRI(T), a novel genus and species, Microbacter margulisiae gen. nov., sp. nov., is proposed. The type strain is ADRI(T) ( = JCM 19374(T) = DSM 27471(T)). |
| 巻・号 | 64(Pt 12) |
| ページ | 3936-3942 |
| 公開日 | 2014-12-1 |
| DOI | 10.1099/ijs.0.066241-0 |
| PMID | 25201913 |
| MeSH | Acids Bacterial Typing Techniques Bacteroidetes / classification* Bacteroidetes / genetics Bacteroidetes / isolation & purification Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Geologic Sediments / microbiology* Molecular Sequence Data Phylogeny* Ponds / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Spain Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| IF | 2.415 |
| 引用数 | 11 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 19374 |