| RRC ID |
34453
|
| Author |
Miyazaki M, Sakai S, Ritalahti KM, Saito Y, Yamanaka Y, Saito Y, Tame A, Uematsu K, Löffler FE, Takai K, Imachi H.
|
| Title |
Sphaerochaeta multiformis sp. nov., an anaerobic, psychrophilic bacterium isolated from subseafloor sediment, and emended description of the genus Sphaerochaeta.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
An anaerobic, psychrophilic bacterium, strain MO-SPC2(T), was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediments collected from off the Shimokita Peninsula of Japan in the north-western Pacific Ocean. Cells were pleomorphic: spherical, annular, curved rod, helical and coccoid cell morphologies were observed. Motility only occurred in helical cells. Strain MO-SPC2(T) grew at 0-17 °C (optimally at 9 °C), at pH 6.0-8.0 (optimally at pH 6.8-7.2) and in 20-40 g NaCl l(-1) (optimally at 20-30 NaCl l(-1)). The strain grew chemo-organotrophically with mono-, di- and polysaccharides. The major end products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. The abundant polar lipids of strain MO-SPC2(T) were phosphatidylglycolipids, phospholipids and glycolipids. The major cellular fatty acids were C14 : 0, C16 : 0 and C16 : 1ω9. Isoprenoid quinones were not detected. The G+C content of the DNA was 32.3 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-SPC2(T) was affiliated with the genus Sphaerochaeta within the phylum Spirochaetes, and its closest relatives were Sphaerochaeta pleomorpha Grapes(T) (88.4 % sequence identity), Sphaerochaeta globosa Buddy(T) (86.7 %) and Sphaerochaeta coccoides SPN1(T) (85.4 %). Based on phenotypic characteristics and phylogenetic traits, strain MO-SPC2(T) is considered to represent a novel species of the genus Sphaerochaeta, for which the name Sphaerochaeta multiformis sp. nov. is proposed. The type strain is MO-SPC2(T) ( = JCM 17281(T) = DSM 23952(T)). An emended description of the genus Sphaerochaeta is also proposed.
|
| Volume |
64(Pt 12)
|
| Pages |
4147-4154
|
| Published |
2014-12-1
|
| DOI |
10.1099/ijs.0.068148-0
|
| PMID |
25249566
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Geologic Sediments / microbiology*
Japan
Molecular Sequence Data
Pacific Ocean
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seawater / microbiology
Sequence Analysis, DNA
Spirochaetaceae / classification*
Spirochaetaceae / genetics
Spirochaetaceae / isolation & purification
|
| IF |
2.415
|
| Times Cited |
15
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 17281 |
