RRC ID 34507
著者 Lee S, Jia B, Pham BP, Shao Y, Kwak JM, Cheong GW.
タイトル Architecture and characterization of sarcosine oxidase from Thermococcus kodakarensis KOD1.
ジャーナル Extremophiles
Abstract Sarcosine oxidase (SOX) catalyzes the oxidation of the methyl group in sarcosine and transfer of the oxidized methyl group into the one-carbon metabolic pool. Here, we separately cloned and expressed α and β subunit of SOX from Thermococcus kodakarensis KOD1 (TkSOX) in Escherichia coli and the recombinant proteins were purified to homogeneity. Gel filtration chromatography and transmission electron microscopy analysis showed that the α subunit formed a dimeric structure and behaved as an NADH dehydrogenase; β subunit was a tetramer that had sarcosine oxidase and L: -proline dehydrogenase activity. The TkSOX complex assembled into the hetero-octameric (αβ)(4) form and had NADH dehydrogenase activity. Gold-label analysis indicated that α and β subunits were oriented in the alternative form. Based on these results, we suggested that TkSOX was a multifunctional enzyme and that each subunit and (αβ)(4) complex may separately exist as a function enzyme in different conditions.
巻・号 16(1)
ページ 87-93
公開日 2012-1-1
DOI 10.1007/s00792-011-0408-x
PMID 22083128
MeSH Base Sequence Biocatalysis Chromatography, Gel DNA Primers Microscopy, Electron Polymerase Chain Reaction Sarcosine Oxidase / chemistry Sarcosine Oxidase / metabolism* Thermococcus / enzymology*
IF 2.462
引用数 9
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 12380