RRC ID 34543
Author Nishimura T, Sato T, Yamamoto Y, Watakabe I, Ohkawa Y, Suyama M, Kobayashi S, Tanaka M.
Title Sex determination. foxl3 is a germ cell-intrinsic factor involved in sperm-egg fate decision in medaka.
Journal Science
Abstract Sex determination is an essential step in the commitment of a germ cell to a sperm or egg. However, the intrinsic factors that determine the sexual fate of vertebrate germ cells are unknown. Here, we show that foxl3, which is expressed in germ cells but not somatic cells in the gonad, is involved in sperm-egg fate decision in medaka fish. Adult XX medaka with disrupted foxl3 developed functional sperm in the expanded germinal epithelium of a histologically functional ovary. In chimeric medaka, mutant germ cells initiated spermatogenesis in female wild-type gonad. These results indicate that a germ cell-intrinsic cue for the sperm-egg fate decision is present in medaka and that spermatogenesis can proceed in a female gonadal environment.
Volume 349(6245)
Pages 328-31
Published 2015-7-17
DOI 10.1126/science.aaa2657
PII science.aaa2657
PMID 26067255
MeSH Animals Female Forkhead Transcription Factors / genetics Forkhead Transcription Factors / physiology* Male Oocytes / cytology Oocytes / physiology* Oryzias / genetics Oryzias / growth & development* Sex Determination Processes / genetics* Spermatogenesis / genetics Spermatozoa / cytology Spermatozoa / physiology*
IF 41.846
Times Cited 52
Medaka cDNAs BAC and fosmids clones