| RRC ID |
3461
|
| Author |
Tsukioka H, Takahashi M, Mon H, Okano K, Mita K, Shimada T, Lee JM, Kawaguchi Y, Koga K, Kusakabe T.
|
| Title |
Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
The argonaute protein family provides central components for RNA interference (RNAi) and related phenomena in a wide variety of organisms. Here, we isolated, from a Bombyx mori cell, a cDNA clone named BmAGO2, which is homologous to Drosophila ARGONAUTE2, the gene encoding a repressive factor for the recombination repair of extrachromosomal double-strand breaks (DSBs). RNAi-mediated silencing of the BmAGO2 sequence markedly increased homologous recombination (HR) repair of DSBs in episomal DNA, but had no effect on that in chromosomes. Moreover, we found that RNAi for BmAGO2 enhanced the integration of linearized DNA into a silkworm chromosome via HR. These results suggested that BmAgo2 protein plays an indispensable role in the repression of extrachromosomal DSB repair.
|
| Volume |
34(4)
|
| Pages |
1092-101
|
| Published |
2006-1-1
|
| DOI |
10.1093/nar/gkj507
|
| PII |
34/4/1092
|
| PMID |
16478716
|
| PMC |
PMC1368654
|
| MeSH |
Amino Acid Sequence
Animals
Argonaute Proteins
Bombyx / cytology
Bombyx / genetics*
Bombyx / metabolism
Cells, Cultured
Chromosomes / metabolism
Cloning, Molecular
DNA Damage
DNA Repair*
Drosophila Proteins / chemistry
Expressed Sequence Tags
Genes, Insect
Insect Proteins / chemistry
Insect Proteins / genetics
Insect Proteins / physiology*
RNA Interference
RNA, Messenger / metabolism
RNA-Induced Silencing Complex / chemistry
RNA-Induced Silencing Complex / genetics
RNA-Induced Silencing Complex / physiology*
Recombination, Genetic
Sequence Homology, Amino Acid
|
| IF |
11.502
|
| Times Cited |
16
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
EST database |
