| RRC ID |
34768
|
| 著者 |
Omori Y, Chaya T, Yoshida S, Irie S, Tsujii T, Furukawa T.
|
| タイトル |
Identification of G Protein-Coupled Receptors (GPCRs) in Primary Cilia and Their Possible Involvement in Body Weight Control.
|
| ジャーナル |
PLoS One
|
| Abstract |
Primary cilia are sensory organelles that harbor various receptors such as G protein-coupled receptors (GPCRs). We analyzed subcellular localization of 138 non-odorant GPCRs. We transfected GPCR expression vectors into NIH3T3 cells, induced ciliogenesis by serum starvation, and observed subcellular localization of GPCRs by immunofluorescent staining. We found that several GPCRs whose ligands are involved in feeding behavior, including prolactin-releasing hormone receptor (PRLHR), neuropeptide FF receptor 1 (NPFFR1), and neuromedin U receptor 1 (NMUR1), localized to the primary cilia. In addition, we found that a short form of dopamine receptor D2 (DRD2S) is efficiently transported to the primary cilia, while a long form of dopamine receptor D2 (DRD2L) is rarely transported to the primary cilia. Using an anti-Prlhr antibody, we found that Prlhr localized to the cilia on the surface of the third ventricle in the vicinity of the hypothalamic periventricular nucleus. We generated the Npy2r-Cre transgenic mouse line in which Cre-recombinase is expressed under the control of the promoter of Npy2r encoding a ciliary GPCR. By mating Npy2r-Cre mice with Ift80 flox mice, we generated Ift80 conditional knockout (CKO) mice in which Npy2r-positive cilia were diminished in number. We found that Ift80 CKO mice exhibited a body weight increase. Our results suggest that Npy2r-positive cilia are important for body weight control.
|
| 巻・号 |
10(6)
|
| ページ |
e0128422
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0128422
|
| PII |
PONE-D-15-05654
|
| PMID |
26053317
|
| PMC |
PMC4459993
|
| MeSH |
Animals
Body Weight*
Cilia / metabolism*
Hypothalamus / metabolism
Integrases / metabolism
Mice
Mice, Knockout
NIH 3T3 Cells
Protein Isoforms / metabolism
Protein Transport
Receptors, Dopamine D2 / metabolism
Receptors, G-Protein-Coupled / metabolism*
Subcellular Fractions / metabolism
Weight Gain
|
| IF |
2.74
|
| 引用数 |
17
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 実験動物マウス |
RBRC01835 |
