RRC ID 34778
Author Ogawa Y, Eto A, Miyake C, Tsuchida N, Miyake H, Takaku Y, Hagiwara H, Oishi K.
Title Induced Pluripotent Stem Cells Generated from P0-Cre;Z/EG Transgenic Mice.
Journal PLoS One
Abstract Neural crest (NC) cells are a migratory, multipotent cell population that arises at the neural plate border, and migrate from the dorsal neural tube to their target tissues, where they differentiate into various cell types. Abnormal development of NC cells can result in severe congenital birth defects. Because only a limited number of cells can be obtained from an embryo, mechanistic studies are difficult to perform with directly isolated NC cells. Protein zero (P0) is expressed by migrating NC cells during the early embryonic period. In the P0-Cre;Z/EG transgenic mouse, transient activation of the P0 promoter induces Cre-mediated recombination, indelibly tagging NC-derived cells with enhanced green fluorescent protein (EGFP). Induced pluripotent stem cell (iPSC) technology offers new opportunities for both mechanistic studies and development of stem cell-based therapies. Here, we report the generation of iPSCs from the P0-Cre;Z/EG mouse. P0-Cre;Z/EG mouse-derived iPSCs (P/G-iPSCs) exhibited pluripotent stem cell properties. In lineage-directed differentiation studies, P/G-iPSCs were efficiently differentiated along the neural lineage while expressing EGFP. These results suggest that P/G-iPSCs are useful to study NC development and NC-associated diseases.
Volume 10(9)
Pages e0138620
Published 2015-1-1
DOI 10.1371/journal.pone.0138620
PII PONE-D-15-29170
PMID 26382630
PMC PMC4575135
MeSH Animals Cell Differentiation* Cell Lineage / genetics Embryo, Mammalian / cytology Embryo, Mammalian / embryology Embryo, Mammalian / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunohistochemistry Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Lac Operon / genetics Mice, Inbred BALB C Mice, Nude Mice, Transgenic Microscopy, Confocal Microscopy, Fluorescence Multipotent Stem Cells / cytology* Multipotent Stem Cells / metabolism Myelin P0 Protein / genetics Myelin P0 Protein / metabolism Neural Crest / cytology* Neural Crest / embryology Neural Crest / metabolism
IF 2.74
Times Cited 1
Mice RBRC01459