RRC ID 34779
Author Isogawa M, Higuchi M, Takahashi M, Oie M, Mori N, Tanaka Y, Aoyagi Y, Fujii M.
Title Rearranged NF-kappa B2 gene in an adult T-cell leukemia cell line.
Journal Cancer Sci.
Abstract Adult T-cell leukemia (ATL) is an aggressive type of leukemia, originating from T-cells infected with human T-cell leukemia virus type 1. Accumulating evidence suggests the aberrant activation of NF-kappaB to be a causative factor mediating the abnormal proliferation of leukemic cells, thus resulting in the development of ATL. A rearranged NF-kappa B2/p100 gene was isolated from an ATL-derived cell line, which was generated by a chromosomal translocation. The isolated NF-kappa B2 mutant is fused with the with no (lysine) deficient protein kinase 1 gene, coding for a 58 kDa protein that retains the DNA binding Rel homology domain, but it lacks the entire ankyrin repeat inhibitory domain, thus suggesting its constitutive activation. This rearranged NF-kappa B2 gene product (p58) was localized in the nucleus, and formed a complex with NF-kappaB p65 or RelB. Moreover, a T-cell line expressing p58 increased the amount of an NF-kappa B2-inducible gene, NF-kappa B2/p100 by itself. These results suggest that such NF-kappa B2 gene rearrangement may therefore be a factor in the constitutive activation of NF-kappaB in ATL, and thereby playing a role in the ATL pathogenesis.
Volume 99(4)
Pages 792-8
Published 2008-4
DOI 10.1111/j.1349-7006.2008.00750.x
PII CAS750
PMID 18377428
MeSH Animals Base Sequence Cell Line, Tumor DNA Mutational Analysis Dimerization Gene Library Humans I-kappa B Kinase / metabolism Leukemia-Lymphoma, Adult T-Cell / genetics* Mice Mutation NF-kappa B p52 Subunit / analysis NF-kappa B p52 Subunit / genetics* NF-kappa B p52 Subunit / metabolism Transcription Factor RelA / metabolism Transcription Factor RelB / metabolism Translocation, Genetic*
IF 4.372
Times Cited 9
WOS Category ONCOLOGY
Resource
DNA material CSII-EF-MCS (RDB04378) CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)