| RRC ID |
34779
|
| Author |
Isogawa M, Higuchi M, Takahashi M, Oie M, Mori N, Tanaka Y, Aoyagi Y, Fujii M.
|
| Title |
Rearranged NF-kappa B2 gene in an adult T-cell leukemia cell line.
|
| Journal |
Cancer Sci
|
| Abstract |
Adult T-cell leukemia (ATL) is an aggressive type of leukemia, originating from T-cells infected with human T-cell leukemia virus type 1. Accumulating evidence suggests the aberrant activation of NF-kappaB to be a causative factor mediating the abnormal proliferation of leukemic cells, thus resulting in the development of ATL. A rearranged NF-kappa B2/p100 gene was isolated from an ATL-derived cell line, which was generated by a chromosomal translocation. The isolated NF-kappa B2 mutant is fused with the with no (lysine) deficient protein kinase 1 gene, coding for a 58 kDa protein that retains the DNA binding Rel homology domain, but it lacks the entire ankyrin repeat inhibitory domain, thus suggesting its constitutive activation. This rearranged NF-kappa B2 gene product (p58) was localized in the nucleus, and formed a complex with NF-kappaB p65 or RelB. Moreover, a T-cell line expressing p58 increased the amount of an NF-kappa B2-inducible gene, NF-kappa B2/p100 by itself. These results suggest that such NF-kappa B2 gene rearrangement may therefore be a factor in the constitutive activation of NF-kappaB in ATL, and thereby playing a role in the ATL pathogenesis.
|
| Volume |
99(4)
|
| Pages |
792-8
|
| Published |
2008-4-1
|
| DOI |
10.1111/j.1349-7006.2008.00750.x
|
| PII |
CAS750
|
| PMID |
18377428
|
| MeSH |
Animals
Base Sequence
Cell Line, Tumor
DNA Mutational Analysis
Dimerization
Gene Library
Humans
I-kappa B Kinase / metabolism
Leukemia-Lymphoma, Adult T-Cell / genetics*
Mice
Mutation
NF-kappa B p52 Subunit / analysis
NF-kappa B p52 Subunit / genetics*
NF-kappa B p52 Subunit / metabolism
Transcription Factor RelA / metabolism
Transcription Factor RelB / metabolism
Translocation, Genetic*
|
| IF |
4.966
|
| Times Cited |
10
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| DNA material |
CSII-EF-MCS (RDB04378)
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
