| RRC ID |
34784
|
| Author |
Takeda H, Inoue H, Kutsuna T, Matsushita N, Takahashi T, Watanabe S, Higashiyama S, Yamamoto H.
|
| Title |
Activation of epidermal growth factor receptor gene is involved in transforming growth factor-beta-mediated fibronectin expression in a chondrocyte progenitor cell line.
|
| Journal |
Int J Mol Med
|
| Abstract |
Chondrocytes produce many types of ECM to maintain elasticity and plasticity in articular cartilage of revolute joints. Both transforming growth factor beta (TGF-beta) and bone morphogenetic proteins (BMPs) induce extracellular matrix proteins such as type IIalpha1 collagen and aggrecan during chondrogenic differentiation in vitro. However, differences in the matrix gene expression pattern by the stimulation of TGF-betas and BMPs remains unclear. In the present study, we created a customized PCR-based ECM array to investigate the pattern of ECM expression genes in the chondrocyte progenitor cell line ATDC5, that was stimulated by TGF-betas or BMPs. Fibronectin (Fn) expression was drastically induced after TGF-beta stimulation, but not BMP-4. Epidermal growth factor receptor (Egfr) gene was also significantly activated in TGF-beta1-induced chondrogenic differentiation as compared to BMP-4-mediated differentiation. Furthermore, EGFR-knockdown assay of the cells showed decreasing Fn expression during TGF-beta1-induced chondrogenic differentiation. These data indicated that Egfr gene activation by TGF-beta is involved in the differences in the expression of cellular matrix genes such as Fn, as compared to the expression pattern induced by BMPs.
|
| Volume |
25(4)
|
| Pages |
593-600
|
| Published |
2010-4-1
|
| DOI |
10.3892/ijmm_00000381
|
| PMID |
20198308
|
| MeSH |
Animals
Bone Morphogenetic Protein 4 / pharmacology
Cell Differentiation / drug effects
Cell Differentiation / genetics
Cell Line
Chondrocytes / cytology*
Chondrocytes / drug effects
Chondrocytes / metabolism*
Down-Regulation / drug effects
ErbB Receptors / genetics*
ErbB Receptors / metabolism
Extracellular Matrix / drug effects
Extracellular Matrix / genetics
Extracellular Matrix / metabolism
Fibronectins / genetics*
Fibronectins / metabolism
Gene Expression Profiling
Gene Knockdown Techniques
Humans
Mice
RNA, Small Interfering / metabolism
Stem Cells / drug effects
Stem Cells / metabolism*
Transcriptional Activation* / drug effects
Transforming Growth Factor beta / pharmacology*
Up-Regulation / drug effects
|
| IF |
3.098
|
| Times Cited |
14
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| DNA material |
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
| Human and Animal Cells |
ATDC5(RCB0565) |
