論文 - 詳細
| RRC ID | 34787 |
|---|---|
| 著者 | Kobayashi K, Takahashi H, Inoue A, Harada H, Toshimori S, Kobayashi Y, Goto K, Sugimoto K, Yano H, Ohnishi T, Tanaka J. |
| タイトル | Oct-3/4 promotes migration and invasion of glioblastoma cells. |
| ジャーナル | J Cell Biochem |
| Abstract |
As a result of increased glioblastoma migration and invasion into normal brain parenchyma, treatment of local tumor recurrence following initial treatment in glioblastoma patients remains challenging. Recent studies have demonstrated increased Oct-3/4 expression, a self-renewal regulator in stem cells, in glioblastomas. However, little is known regarding the influence of Oct-3/4 in glioblastoma cell invasiveness. The present study established Oct-3/4-overexpressing glioblastoma cells, which were prepared from human glioblastoma patients, to assess migration, invasion, and mRNA expression profiles of integrins and matrix metalloproteinases (MMPs). Compared with control cells, Oct-3/4 expressing-glioblastoma cells exhibited increased migration and invasion in wound healing and Matrigel invasion assays. Oct-3/4 overexpression resulted in upregulated FAK and c-Src expression, which mediate integrin signals. Vinculin accumulated along the leading edges of Oct-3/4 expressing-glioblastoma cells and associated with membrane ruffles during cell migration. Oct-3/4 expressing-cells exhibited increased MMP-13 mRNA expression and MMP-13 knockdown by shRNA suppressed cell invasion into Matrigel and organotypic brain slices. These results suggested that Oct-3/4 enhanced degradation of surrounding extracellular matrix by increasing MMP-13 expression and altering integrin signaling. Therefore, Oct-3/4 might contribute to tumor promoting activity in glioblastomas. |
| 巻・号 | 113(2) |
| ページ | 508-17 |
| 公開日 | 2012-2-1 |
| DOI | 10.1002/jcb.23374 |
| PMID | 21938739 |
| MeSH | Actin Cytoskeleton / metabolism Animals Brain / pathology Brain Neoplasms / metabolism Brain Neoplasms / pathology* Cell Movement* Focal Adhesions / metabolism Gene Expression Gene Expression Regulation, Neoplastic Glioblastoma / metabolism Glioblastoma / pathology* Humans Integrins / genetics Integrins / metabolism Matrix Metalloproteinase 13 / genetics Matrix Metalloproteinase 13 / metabolism Neoplasm Invasiveness Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism* Rats Rats, Wistar Tissue Culture Techniques Tumor Cells, Cultured Up-Regulation |
| IF | 4.237 |
| 引用数 | 28 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |