論文 - 詳細
| RRC ID | 34798 |
|---|---|
| 著者 | Stamper EL, Rodenbusch SE, Rosu S, Ahringer J, Villeneuve AM, Dernburg AF. |
| タイトル | Identification of DSB-1, a protein required for initiation of meiotic recombination in Caenorhabditis elegans, illuminates a crossover assurance checkpoint. |
| ジャーナル | PLoS Genet |
| Abstract |
Meiotic recombination, an essential aspect of sexual reproduction, is initiated by programmed DNA double-strand breaks (DSBs). DSBs are catalyzed by the widely-conserved Spo11 enzyme; however, the activity of Spo11 is regulated by additional factors that are poorly conserved through evolution. To expand our understanding of meiotic regulation, we have characterized a novel gene, dsb-1, that is specifically required for meiotic DSB formation in the nematode Caenorhabditis elegans. DSB-1 localizes to chromosomes during early meiotic prophase, coincident with the timing of DSB formation. DSB-1 also promotes normal protein levels and chromosome localization of DSB-2, a paralogous protein that plays a related role in initiating recombination. Mutations that disrupt crossover formation result in prolonged DSB-1 association with chromosomes, suggesting that nuclei may remain in a DSB-permissive state. Extended DSB-1 localization is seen even in mutants with defects in early recombination steps, including spo-11, suggesting that the absence of crossover precursors triggers the extension. Strikingly, failure to form a crossover precursor on a single chromosome pair is sufficient to extend the localization of DSB-1 on all chromosomes in the same nucleus. Based on these observations we propose a model for crossover assurance that acts through DSB-1 to maintain a DSB-permissive state until all chromosome pairs acquire crossover precursors. This work identifies a novel component of the DSB machinery in C. elegans, and sheds light on an important pathway that regulates DSB formation for crossover assurance. |
| 巻・号 | 9(8) |
| ページ | e1003679 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pgen.1003679 |
| PII | PGENETICS-D-13-00682 |
| PMID | 23990794 |
| PMC | PMC3749324 |
| MeSH | Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics* Cell Cycle Checkpoints / genetics* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Chromosomal Proteins, Non-Histone / genetics* Chromosome Pairing / genetics Chromosome Segregation / genetics Chromosomes / metabolism Crossing Over, Genetic DNA Breaks, Double-Stranded* Homologous Recombination / genetics* Meiosis / genetics* Mutation |
| IF | 5.175 |
| 引用数 | 40 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5034 tm1268 tm611 tm574 tm3655 |