RRC ID |
34806
|
Author |
Narita Y, Sugimoto A, Kawashima D, Watanabe T, Kanda T, Kimura H, Tsurumi T, Murata T.
|
Title |
A Herpesvirus Specific Motif of Epstein-Barr Virus DNA Polymerase Is Required for the Efficient Lytic Genome Synthesis.
|
Journal |
Sci Rep
|
Abstract |
Epstein-Barr virus (EBV) is associated with several malignancies, including Burkitt lymphoma and nasopharyngeal carcinoma. To overcome such disorders, understanding the molecular mechanisms of the EBV replication is important. The EBV DNA polymerase (Pol) is one of the essential factors for viral lytic DNA replication. Although it is well known that its C-terminal half, possessing DNA polymerase and 3'-5' exonuclease activity, is highly conserved among Family B Pols, the NH2-terminal half has yet to be characterized in detail. In this study, we show that a stretch of hydrophobic amino acids within the pre-NH2-terminal domain of EBV Pol plays important role. In addition, we could identify the most essential residue for replication in the motif. These findings will shed light on molecular mechanisms of viral DNA synthesis and will help to develop new herpesviruses treatments.
|
Volume |
5
|
Pages |
11767
|
Published |
2015-6-30
|
DOI |
10.1038/srep11767
|
PII |
srep11767
|
PMID |
26123572
|
PMC |
PMC4485236
|
MeSH |
Amino Acid Sequence
Conserved Sequence
DNA Replication
DNA, Viral / genetics
DNA, Viral / metabolism
DNA-Directed DNA Polymerase / chemistry*
DNA-Directed DNA Polymerase / metabolism
Genome, Viral
HEK293 Cells
Herpesvirus 4, Human / enzymology*
Herpesvirus 4, Human / genetics
Humans
Hydrophobic and Hydrophilic Interactions
Molecular Sequence Data
Viral Proteins / chemistry*
Viral Proteins / metabolism
Virus Replication*
|
IF |
3.998
|
Times Cited |
4
|
WOS Category
|
VIROLOGY
|
Resource |
DNA material |
pcDNA3.1(-) BALF5 (RDB13542)
pcDNA3.1(-) BALF5 hsm 6A (RDB13543)
pcDNA3.1(-) FLAG-BALF5 (RDB13544)
pcDNA3.1(-) FLAG-BALF5 hsm 6A (RDB13545)
pcDNA3.1(-) HA-BSLF1 (RDB13546)
pcDNA3.1(-) HA-BSLF2/3 (RDB13547)
pcDNA3.1(-) HA-BSLF4 (RDB13548). |