Reference - Detail
| RRC ID | 34807 |
|---|---|
| Author | Huang C, Seino J, Wang L, Haga Y, Suzuki T. |
| Title | Autophagy regulates the stability of sialin, a lysosomal sialic acid transporter. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Macroautophagy plays a critical role in catabolizing cytosolic components via lysosomal degradation. Recent findings from our studies indicate that basal autophagy is required for the efficient lysosomal catabolism of sialyloligosaccharides, and that the downregulation of sialin, a lysosomal transporter of sialic acids can cause a significant delay in the cytosolic accumulation of such glycans. The findings reported herein show that the sialin protein level was increased when the autophagy process was inhibited. This effect appears to be specific to sialin, since the amount of LAMP1, another lysosomal membrane protein, remains constant under the same conditions. Our results suggest that autophagy may regulate the stability of sialin, and it could lead to the cytosolic accumulation of sialyloligosaccharides in autophagy-defective cells. |
| Volume | 79(4) |
| Pages | 553-7 |
| Published | 2015-1-1 |
| DOI | 10.1080/09168451.2014.991682 |
| PMID | 25494612 |
| MeSH | Animals Autophagy / genetics* Autophagy-Related Protein 5 Biological Transport Cell Line Fibroblasts / cytology Fibroblasts / metabolism* Gene Expression Regulation Lysosome-Associated Membrane Glycoproteins / genetics Lysosome-Associated Membrane Glycoproteins / metabolism Lysosomes / metabolism* Mice Microtubule-Associated Proteins / deficiency Microtubule-Associated Proteins / genetics Oligosaccharides / metabolism Organic Anion Transporters / chemistry Organic Anion Transporters / genetics Organic Anion Transporters / metabolism* Protein Stability Sialic Acids / metabolism* Signal Transduction Symporters / chemistry Symporters / genetics Symporters / metabolism* |
| IF | 1.516 |
| Times Cited | 3 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED |
| Resource | |
| DNA material | pENTR-sialin (RDB13483) pcDNA6.2/V5-DEST-sialin (RDB13484). |