論文 - 詳細
| RRC ID | 34842 |
|---|---|
| 著者 | Nanbu T, Nguyễn LC, Habib AG, Hirata N, Ukimori S, Tanaka D, Masuda K, Takahashi K, Yukawa M, Tsuchiya E, Ueno M. |
| タイトル | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. |
| ジャーナル | PLoS One |
| Abstract |
The uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromosome fusion in S. pombe. However, the factors responsible for the resection of uncapped telomeres remain unknown. In this study, we investigated these factors and found that concomitant deletion of rqh1+ and exo1+ alleviated the loss of telomeres following Pot1 shut-off, suggesting that Rqh1 and Exo1 are redundantly involved in the resection of uncapped telomeres. We also investigated the role of Rqh1 helicase activity and found it to be essential for the resection of uncapped telomeres. Moreover, we found that Dna2 and Exo1 function redundantly in the resection of uncapped telomeres. Taken together, these results suggest that Exo1 and Rqh1-Dna2 redundantly contribute to the resection of uncapped telomeres. Therefore, our results demonstrate that nmt-pot1-aid is an important model strain to study the role of helicases and nucleases in the resection of uncapped telomeres and to improve our understanding of DNA double-strand break repair. |
| 巻・号 | 10(10) |
| ページ | e0140456 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0140456 |
| PII | PONE-D-14-40634 |
| PMID | 26465752 |
| PMC | PMC4605587 |
| MeSH | Chromosomes, Fungal / genetics Chromosomes, Fungal / metabolism DNA Helicases / genetics* DNA Helicases / metabolism Exodeoxyribonucleases / genetics* Exodeoxyribonucleases / metabolism Flap Endonucleases / genetics* Flap Endonucleases / metabolism Gene Deletion Microbial Viability / genetics Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Shelterin Complex Telomere / genetics* Telomere / metabolism Telomere-Binding Proteins / genetics |
| IF | 2.74 |
| 引用数 | 7 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY7512(JY741) |