| RRC ID |
34842
|
| 著者 |
Nanbu T, Nguyễn LC, Habib AG, Hirata N, Ukimori S, Tanaka D, Masuda K, Takahashi K, Yukawa M, Tsuchiya E, Ueno M.
|
| タイトル |
Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres.
|
| ジャーナル |
PLoS One
|
| Abstract |
The uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromosome fusion in S. pombe. However, the factors responsible for the resection of uncapped telomeres remain unknown. In this study, we investigated these factors and found that concomitant deletion of rqh1+ and exo1+ alleviated the loss of telomeres following Pot1 shut-off, suggesting that Rqh1 and Exo1 are redundantly involved in the resection of uncapped telomeres. We also investigated the role of Rqh1 helicase activity and found it to be essential for the resection of uncapped telomeres. Moreover, we found that Dna2 and Exo1 function redundantly in the resection of uncapped telomeres. Taken together, these results suggest that Exo1 and Rqh1-Dna2 redundantly contribute to the resection of uncapped telomeres. Therefore, our results demonstrate that nmt-pot1-aid is an important model strain to study the role of helicases and nucleases in the resection of uncapped telomeres and to improve our understanding of DNA double-strand break repair.
|
| 巻・号 |
10(10)
|
| ページ |
e0140456
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0140456
|
| PII |
PONE-D-14-40634
|
| PMID |
26465752
|
| PMC |
PMC4605587
|
| MeSH |
Chromosomes, Fungal / genetics
Chromosomes, Fungal / metabolism
DNA Helicases / genetics*
DNA Helicases / metabolism
Exodeoxyribonucleases / genetics*
Exodeoxyribonucleases / metabolism
Flap Endonucleases / genetics*
Flap Endonucleases / metabolism
Gene Deletion
Microbial Viability / genetics
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
Shelterin Complex
Telomere / genetics*
Telomere / metabolism
Telomere-Binding Proteins / genetics
|
| IF |
2.74
|
| 引用数 |
7
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 酵母 |
FY7512(JY741) |
