Reference - Detail
| RRC ID | 34879 |
|---|---|
| Author | Kanoh H, Kuraishi T, Tong LL, Watanabe R, Nagata S, Kurata S. |
| Title | Ex vivo genome-wide RNAi screening of the Drosophila Toll signaling pathway elicited by a larva-derived tissue extract. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Damage-associated molecular patterns (DAMPs), so-called "danger signals," play important roles in host defense and pathophysiology in mammals and insects. In Drosophila, the Toll pathway confers damage responses during bacterial infection and improper cell-fate control. However, the intrinsic ligands and signaling mechanisms that potentiate innate immune responses remain unknown. Here, we demonstrate that a Drosophila larva-derived tissue extract strongly elicits Toll pathway activation via the Toll receptor. Using this extract, we performed ex vivo genome-wide RNAi screening in Drosophila cultured cells, and identified several signaling factors that are required for host defense and antimicrobial-peptide expression in Drosophila adults. These results suggest that our larva-derived tissue extract contains active ingredients that mediate Toll pathway activation, and the screening data will shed light on the mechanisms of damage-related Toll pathway signaling in Drosophila. |
| Volume | 467(2) |
| Pages | 400-6 |
| Published | 2015-11-13 |
| DOI | 10.1016/j.bbrc.2015.09.138 |
| PII | S0006-291X(15)30654-9 |
| PMID | 26427875 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / immunology Animals Antigens, Differentiation / genetics Antigens, Differentiation / immunology Antimicrobial Cationic Peptides / biosynthesis Antimicrobial Cationic Peptides / immunology* Cells, Cultured Drosophila Proteins / genetics Drosophila Proteins / immunology Drosophila melanogaster / genetics Drosophila melanogaster / immunology* Drosophila melanogaster / microbiology Gene Expression Regulation Genes, Reporter High-Throughput Screening Assays Immunity, Innate Larva / chemistry Larva / genetics Larva / immunology Larva / microbiology Luciferases / genetics Luciferases / metabolism Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / immunology RNA Interference Receptors, Immunologic / genetics Receptors, Immunologic / immunology Signal Transduction / immunology* Staphylococcus saprophyticus / immunology Tissue Extracts / chemistry Tissue Extracts / immunology Toll-Like Receptors / genetics Toll-Like Receptors / immunology* |
| IF | 2.985 |
| Times Cited | 9 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Drosophila | |