RRC ID 34879
Author Kanoh H, Kuraishi T, Tong LL, Watanabe R, Nagata S, Kurata S.
Title Ex vivo genome-wide RNAi screening of the Drosophila Toll signaling pathway elicited by a larva-derived tissue extract.
Journal Biochem Biophys Res Commun
Abstract Damage-associated molecular patterns (DAMPs), so-called "danger signals," play important roles in host defense and pathophysiology in mammals and insects. In Drosophila, the Toll pathway confers damage responses during bacterial infection and improper cell-fate control. However, the intrinsic ligands and signaling mechanisms that potentiate innate immune responses remain unknown. Here, we demonstrate that a Drosophila larva-derived tissue extract strongly elicits Toll pathway activation via the Toll receptor. Using this extract, we performed ex vivo genome-wide RNAi screening in Drosophila cultured cells, and identified several signaling factors that are required for host defense and antimicrobial-peptide expression in Drosophila adults. These results suggest that our larva-derived tissue extract contains active ingredients that mediate Toll pathway activation, and the screening data will shed light on the mechanisms of damage-related Toll pathway signaling in Drosophila.
Volume 467(2)
Pages 400-6
Published 2015-11-13
DOI 10.1016/j.bbrc.2015.09.138
PII S0006-291X(15)30654-9
PMID 26427875
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / immunology Animals Antigens, Differentiation / genetics Antigens, Differentiation / immunology Antimicrobial Cationic Peptides / biosynthesis Antimicrobial Cationic Peptides / immunology* Cells, Cultured Drosophila Proteins / genetics Drosophila Proteins / immunology Drosophila melanogaster / genetics Drosophila melanogaster / immunology* Drosophila melanogaster / microbiology Gene Expression Regulation Genes, Reporter High-Throughput Screening Assays Immunity, Innate Larva / chemistry Larva / genetics Larva / immunology Larva / microbiology Luciferases / genetics Luciferases / metabolism Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / immunology RNA Interference Receptors, Immunologic / genetics Receptors, Immunologic / immunology Signal Transduction / immunology* Staphylococcus saprophyticus / immunology Tissue Extracts / chemistry Tissue Extracts / immunology Toll-Like Receptors / genetics Toll-Like Receptors / immunology*
IF 2.705
Times Cited 9