Reference - Detail
|Author||Yoshida S, Kaido M, Kitajima TS.|
|Title||Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes.|
A model for mitosis suggests that correct kinetochore-microtubule (KT-MT) attachments are stabilized by spatial separation of the attachment sites from Aurora B kinase through sister KT stretching. However, the spatiotemporal regulation of attachment stability during meiosis I (MI) in oocytes remains unclear. Here, we found that in mouse oocytes, Aurora B and C (B/C) are located in close proximity to KT-MT attachment sites after bivalent stretching due to an intrinsic property of the MI chromosomes. The Aurora B/C activity destabilizes correct attachments while allowing a considerable amount of incorrect attachments to form. KT-MT attachments are eventually stabilized through KT dephosphorylation by PP2A-B56 phosphatase, which is progressively recruited to KTs depending on the BubR1 phosphorylation resulting from the timer Cdk1 and independent of bivalent stretching. Thus, oocytes lack a mechanism for coordinating bivalent stretching and KT phosphoregulation during MI, which may explain the high frequency of KT-MT attachment errors.
|MeSH||Animals Aurora Kinase B / metabolism Aurora Kinase C / metabolism CDC2 Protein Kinase / metabolism Cell Cycle Proteins / metabolism Chromosome Segregation* Female Fluorescent Antibody Technique Image Processing, Computer-Assisted Kinetochores / physiology* Meiosis / physiology* Mice Microtubules / physiology* Oocytes / cytology Oocytes / metabolism* Phosphorylation Protein Phosphatase 2 / metabolism Protein-Serine-Threonine Kinases / metabolism|
|WOS Category||DEVELOPMENTAL BIOLOGY CELL BIOLOGY|
|DNA material||pGEMHE-3mEGFP-PP2A-B56epsilon (RDB13509) pGEMHE-3mEGFP-PP2A-B55alpha (RDB13510) pGEMHE-3mEGFP-PP2A-PR72 (RDB13511) pGEMHE-mCcnb1-mEGFP (RDB13512) pGEMHE-mBubR1-WT (RDB13513) pGEMHE-mBubR1-3A (RDB13514) pGEMHE-mBubR1-3D (RDB13515) pGEMHE-mHec1-sfGFP (RDB13516) pGEMHE-Nuf2 (RDB13517) pGEMHE-tdTomato-mCENP-C (RDB13518) pGEMHE-mEGFP-INCENP (RDB13519)|