RRC ID 34912
Author Ferreira ME, Berndt KD, Nilsson J, Wright AP.
Title WD40 domain divergence is important for functional differences between the fission yeast Tup11 and Tup12 co-repressor proteins.
Journal PLoS One
Abstract We have previously demonstrated that subsets of Ssn6/Tup target genes have distinct requirements for the Schizosaccharomyces pombe homologs of the Tup1/Groucho/TLE co-repressor proteins, Tup11 and Tup12. The very high level of divergence in the histone interacting repression domains of the two proteins suggested that determinants distinguishing Tup11 and Tup12 might be located in this domain. Here we have combined phylogenetic and structural analysis as well as phenotypic characterization, under stress conditions that specifically require Tup12, to identify and characterize the domains involved in Tup12-specific action. The results indicate that divergence in the repression domain is not generally relevant for Tup12-specific function. Instead, we show that the more highly conserved C-terminal WD40 repeat domain of Tup12 is important for Tup12-specific function. Surface amino acid residues specific for the WD40 repeat domain of Tup12 proteins in different fission yeasts are clustered in blade 3 of the propeller-like structure that is characteristic of WD40 repeat domains. The Tup11 and Tup12 proteins in fission yeasts thus provide an excellent model system for studying the functional divergence of WD40 repeat domains.
Volume 5(6)
Pages e11009
Published 2010-6-8
DOI 10.1371/journal.pone.0011009
PMID 20544037
PMC PMC2882346
MeSH Repressor Proteins / chemistry Repressor Proteins / genetics Repressor Proteins / physiology* Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / physiology*
IF 2.74
Times Cited 3
Yeast yFS275