RRC ID 35105
Author Auesukaree C, Koedrith P, Saenpayavai P, Asvarak T, Benjaphokee S, Sugiyama M, Kaneko Y, Harashima S, Boonchird C.
Title Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits.
Journal J Biosci Bioeng
Abstract For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723, C3751 and C3867) from Thai fruits were capable of growing and producing 38 g/L ethanol up to 41°C. Based on genetic analyses, these isolates were prototrophic and homothallic, with dominant homothallic and thermotolerant phenotypes. After short-term (30 min) and long-term (12 h) exposure at 37°C, expression levels increased for the heat stress-response genes HSP26, SSA4, HSP82, and HSP104 encoding the heat shock proteins small HSP, HSP70, HSP90 and the HSP100 family, respectively. In isolates C3723 and C3867, expression was significantly higher than that in reference isolates W303 and TISTR5606 for TPS1 encoding trehalose-6-phosphate synthase, NTH1 encoding neutral trehalase and GSY1 encoding glycogen synthase. The results suggested that continuous high expression of heat stress-response genes was important for the long-term, heat stress tolerance of these thermotolerant isolates.
Volume 114(2)
Pages 144-9
Published 2012-8-1
DOI 10.1016/j.jbiosc.2012.03.012
PII S1389-1723(12)00146-6
PMID 22579450
MeSH Ethanol / metabolism Fermentation Fruit / microbiology* Gene Expression Regulation, Fungal / genetics* Genes, Fungal* Glucosyltransferases / genetics Heat-Shock Response / genetics Hot Temperature* Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / isolation & purification* Saccharomyces cerevisiae / physiology Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Transcriptome* Trehalase / genetics Trehalose / biosynthesis Trehalose / metabolism
IF 2.366
Times Cited 24
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Yeast NA