RRC ID 35128
Author Numamoto M, Sasano Y, Hirasaki M, Sugiyama M, Maekawa H, Harashima S.
Title The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiae.
Journal J Biochem
Abstract The Saccharomyces cerevisiae Siw14, a tyrosine phosphatase involved in the response to caffeine, participates in regulation of the phosphorylation and intracellular localization of Gln3, a GATA transcriptional activator of nitrogen catabolite repression-sensitive genes. In Δsiw14 cells, the phosphorylation level of Gln3 is decreased and the nuclear localization of Gln3 is stimulated by caffeine. However, the mechanism by which Siw14 controls the localization and function of Gln3 remains unclear, although the nuclear localization of Gln3 is known to be induced by activation of the type 2A phosphatases (PP2As) Pph21 and Pph22, and the type 2A-related phosphatase Sit4. In this study, we show that the increased nuclear localization of Gln3 in response to caffeine caused by disruption of the SIW14 gene is dependent on the Sit4 and PP2A phosphatases. We also show that decreased phosphorylation of Gln3 caused by disruption of the SIW14 gene is completely suppressed by deletion of both PPH21 and PPH22, but only partially suppressed by deletion of SIT4. Taking these results together, we conclude that Siw14 functions upstream of Pph21 and Pph22 as an inhibitor of the phosphorylation and localization of Gln3, and that Sit4 acts independently of Siw14.
Volume 157(1)
Pages 53-64
Published 2015-1-1
DOI 10.1093/jb/mvu055
PII mvu055
PMID 25313402
MeSH Caffeine / pharmacology Gene Expression Regulation, Fungal / drug effects Phosphorylation / drug effects Protein Phosphatase 2 / biosynthesis* Protein Phosphatase 2 / genetics Protein Tyrosine Phosphatases / biosynthesis* Protein Tyrosine Phosphatases / genetics Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins / biosynthesis* Saccharomyces cerevisiae Proteins / genetics Transcription Factors / biosynthesis* Transcription Factors / genetics
IF 2.476
Times Cited 2
Yeast NA