RRC ID 35158
Author Hosono C, Matsuda R, Adryan B, Samakovlis C.
Title Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Journal Nat Cell Biol
Abstract In contrast to planes, three-dimensional (3D) structures such as tubes are physically anisotropic. Tubular organs exhibit a striking orientation of landmarks according to the physical anisotropy of the 3D shape, in addition to planar cell polarization. However, the influence of 3D tissue topography on the constituting cells remains underexplored. Here, we identify a regulatory network polarizing cellular biochemistry according to the physical anisotropy of the 3D tube geometry (tube cell polarization) by a genome-wide, tissue-specific RNAi screen. During Drosophila airway remodelling, each apical cellular junction is equipotent to establish perpendicular actomyosin cables, irrespective of the longitudinal or transverse tube axis. A dynamic transverse enrichment of atypical protein kinase C (aPKC) shifts the balance and transiently targets activated small GTPase RhoA, myosin phosphorylation and Rab11 vesicle trafficking to longitudinal junctions. We propose that the PAR complex translates tube physical anisotropy into longitudinal junctional anisotropy, where cell-cell communication aligns the contractile cytoskeleton of neighbouring cells.
Volume 17(12)
Pages 1569-76
Published 2015-12-1
DOI 10.1038/ncb3267
PII ncb3267
PMID 26551273
MeSH Actins / genetics Actins / metabolism Airway Remodeling Animals Animals, Genetically Modified Anisotropy Cell Communication Cell Polarity* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / embryology Drosophila melanogaster / genetics Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Female Gene Regulatory Networks Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Intercellular Junctions / metabolism* Larva / cytology Larva / metabolism Male Microscopy, Confocal Myosins / genetics Myosins / metabolism Phosphorylation Protein Kinase C / genetics Protein Kinase C / metabolism RNA Interference Respiratory System / cytology* Respiratory System / embryology Respiratory System / metabolism rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism rhoA GTP-Binding Protein / genetics rhoA GTP-Binding Protein / metabolism
IF 20.042
Times Cited 14
Drosophila 10844R-3 1630R-1 7134R-6 4032R-2 4726R-3 15438R-2 4019R-3 1718R-3 15221R-1 12290R-3 11576R-3 9210R-2 17520R-2 1380R-3 2055R-1 8132R-3 14940R-1 7337R-1 17137R-2 6214R-2 7882R-1 2196R-2 8565R-2 16928R-1 32250R-1 8057R-4 8742R-2 8637R-1 8309R-1 13112R-2 3522R-1 11325R-3 10376R-3 31187R-3 7918R-3 10572R-1 13111R-1 8596R-2 1815R-3 5092R-2 5626R-1 6293R-2 9156R-1 8816R-1 2165R-3 9262R-1 6645R-1 8210R-2 17975R-2 8791R-1 15050R-1 2210R-2 10498R-2 1709R-6 14652R-2 13762R-1 8632R-1 4924R-2 14196R-1 1615R-1 6647R-3 6783R-3 10738R-2 1796R-2 11739R-1 17598R-1 8509R-1 1801R-2 4587R-4 4527R-2 7393R-1 12217R-2 1216R-1 1208R-1 10089R-3 7719R-1 10233R-4 9115R-1 9663R-1 4225R-1 8934R-1 11621R-2 9018R-3 4607R-1 31049R-1 1495R-1 14305R-3