RRC ID 35161
著者 Reimels TA, Pfleger CM.
タイトル Drosophila Rabex-5 restricts Notch activity in hematopoietic cells and maintains hematopoietic homeostasis.
ジャーナル J Cell Sci
Abstract Hematopoietic homeostasis requires the maintenance of a reservoir of undifferentiated blood cell progenitors and the ability to replace or expand differentiated blood cell lineages when necessary. Multiple signaling pathways function in these processes, but how their spatiotemporal control is established and their activity is coordinated in the context of the entire hematopoietic network are still poorly understood. We report here that loss of the gene Rabex-5 in Drosophila causes several hematopoietic abnormalities, including blood cell (hemocyte) overproliferation, increased size of the hematopoietic organ (the lymph gland), lamellocyte differentiation and melanotic mass formation. Hemocyte-specific Rabex-5 knockdown was sufficient to increase hemocyte populations, increase lymph gland size and induce melanotic masses. Rabex-5 negatively regulates Ras, and we show that Ras activity is responsible for specific Rabex-5 hematopoietic phenotypes. Surprisingly, Ras-independent Notch protein accumulation and transcriptional activity in the lymph gland underlie multiple distinct hematopoietic phenotypes of Rabex-5 loss. Thus, Rabex-5 plays an important role in Drosophila hematopoiesis and might serve as an axis coordinating Ras and Notch signaling in the lymph gland.
巻・号 128(24)
ページ 4512-25
公開日 2015-12-15
DOI 10.1242/jcs.174433
PII jcs.174433
PMID 26567216
PMC PMC4696494
MeSH Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Hematopoiesis / physiology* Hematopoietic Stem Cells / cytology Hematopoietic Stem Cells / metabolism* Hemocytes / cytology Hemocytes / metabolism* Receptors, Notch / genetics Receptors, Notch / metabolism* Signal Transduction / physiology* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism*
IF 4.573
引用数 3
WOS 分野 CELL BIOLOGY
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