| RRC ID |
35190
|
| Author |
Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH.
|
| Title |
The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.
|
| Journal |
Cell
|
| Abstract |
The ribosomal elongation cycle describes a series of reactions prolonging the nascent polypeptide chain by one amino acid and driven by two universal elongation factors termed EF-Tu and EF-G in bacteria. Here we demonstrate that the extremely conserved LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis. LepA has the unique function of back-translocating posttranslocational ribosomes, and the results suggest that it recognizes ribosomes after a defective translocation reaction and induces a back-translocation, thus giving EF-G a second chance to translocate the tRNAs correctly. We suggest renaming LepA as elongation factor 4 (EF4).
|
| Volume |
127(4)
|
| Pages |
721-33
|
| Published |
2006-11-17
|
| DOI |
10.1016/j.cell.2006.09.037
|
| PII |
S0092-8674(06)01298-0
|
| PMID |
17110332
|
| MeSH |
Amino Acid Sequence
Computational Biology
Conserved Sequence*
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / metabolism*
GTP Phosphohydrolases / metabolism
Green Fluorescent Proteins / metabolism
Models, Genetic
Molecular Sequence Data
Peptide Elongation Factor G / chemistry
Peptide Elongation Factors / metabolism*
Peptide Initiation Factors
Phylogeny
Protein Biosynthesis / genetics*
Protein Structure, Secondary
Protein Structure, Tertiary
Ribosomes / metabolism*
Transcription, Genetic
Transcriptional Elongation Factors / chemistry
Transcriptional Elongation Factors / metabolism*
|
| IF |
38.637
|
| Times Cited |
150
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
|
