Reference - Detail
| RRC ID | 35190 |
|---|---|
| Author | Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH. |
| Title | The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome. |
| Journal | Cell |
| Abstract |
The ribosomal elongation cycle describes a series of reactions prolonging the nascent polypeptide chain by one amino acid and driven by two universal elongation factors termed EF-Tu and EF-G in bacteria. Here we demonstrate that the extremely conserved LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis. LepA has the unique function of back-translocating posttranslocational ribosomes, and the results suggest that it recognizes ribosomes after a defective translocation reaction and induces a back-translocation, thus giving EF-G a second chance to translocate the tRNAs correctly. We suggest renaming LepA as elongation factor 4 (EF4). |
| Volume | 127(4) |
| Pages | 721-33 |
| Published | 2006-11-17 |
| DOI | 10.1016/j.cell.2006.09.037 |
| PII | S0092-8674(06)01298-0 |
| PMID | 17110332 |
| MeSH | Amino Acid Sequence Computational Biology Conserved Sequence* Escherichia coli / metabolism* Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* GTP Phosphohydrolases / metabolism Green Fluorescent Proteins / metabolism Models, Genetic Molecular Sequence Data Peptide Elongation Factor G / chemistry Peptide Elongation Factors / metabolism* Peptide Initiation Factors Phylogeny Protein Biosynthesis / genetics* Protein Structure, Secondary Protein Structure, Tertiary Ribosomes / metabolism* Transcription, Genetic Transcriptional Elongation Factors / chemistry Transcriptional Elongation Factors / metabolism* |
| IF | 38.637 |
| Times Cited | 150 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |