RRC ID |
35190
|
著者 |
Qin Y, Polacek N, Vesper O, Staub E, Einfeldt E, Wilson DN, Nierhaus KH.
|
タイトル |
The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.
|
ジャーナル |
Cell
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Abstract |
The ribosomal elongation cycle describes a series of reactions prolonging the nascent polypeptide chain by one amino acid and driven by two universal elongation factors termed EF-Tu and EF-G in bacteria. Here we demonstrate that the extremely conserved LepA protein, present in all bacteria and mitochondria, is a third elongation factor required for accurate and efficient protein synthesis. LepA has the unique function of back-translocating posttranslocational ribosomes, and the results suggest that it recognizes ribosomes after a defective translocation reaction and induces a back-translocation, thus giving EF-G a second chance to translocate the tRNAs correctly. We suggest renaming LepA as elongation factor 4 (EF4).
|
巻・号 |
127(4)
|
ページ |
721-33
|
公開日 |
2006-11-17
|
DOI |
10.1016/j.cell.2006.09.037
|
PII |
S0092-8674(06)01298-0
|
PMID |
17110332
|
MeSH |
Amino Acid Sequence
Computational Biology
Conserved Sequence*
Escherichia coli / metabolism*
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / metabolism*
GTP Phosphohydrolases / metabolism
Green Fluorescent Proteins / metabolism
Models, Genetic
Molecular Sequence Data
Peptide Elongation Factor G / chemistry
Peptide Elongation Factors / metabolism*
Peptide Initiation Factors
Phylogeny
Protein Biosynthesis / genetics*
Protein Structure, Secondary
Protein Structure, Tertiary
Ribosomes / metabolism*
Transcription, Genetic
Transcriptional Elongation Factors / chemistry
Transcriptional Elongation Factors / metabolism*
|
IF |
38.637
|
引用数 |
150
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
|