論文 - 詳細
| RRC ID | 35217 |
|---|---|
| 著者 | Umezawa Y, Shimada T, Kori A, Yamada K, Ishihama A. |
| タイトル | The uncharacterized transcription factor YdhM is the regulator of the nemA gene, encoding N-ethylmaleimide reductase. |
| ジャーナル | J Bacteriol |
| Abstract |
N-ethylmaleimide (NEM) has been used as a specific reagent of Cys modification in proteins and thus is toxic for cell growth. On the Escherichia coli genome, the nemA gene coding for NEM reductase is located downstream of the gene encoding an as-yet-uncharacterized transcription factor, YdhM. Disruption of the ydhM gene results in reduction of nemA expression even in the induced state, indicating that the two genes form a single operon. After in vitro genomic SELEX screening, one of the target recognition sequences for YdhM was identified within the promoter region for this ydhM-nemA operon. Both YdhM binding in vitro to the ydhM promoter region and transcription repression in vivo of the ydhM-nemA operon by YdhM were markedly reduced by the addition of NEM. Taken together, we propose that YdhM is the repressor for the nemA gene, thus hereafter designated NemR. The repressor function of NemR was inactivated by the addition of not only NEM but also other Cys modification reagents, implying that Cys modification of NemR renders it inactive. This is an addition to the mode of controlling activity of transcription factors by alkylation with chemical agents. |
| 巻・号 | 190(17) |
| ページ | 5890-7 |
| 公開日 | 2008-9-1 |
| DOI | 10.1128/JB.00459-08 |
| PII | JB.00459-08 |
| PMID | 18567656 |
| PMC | PMC2519536 |
| MeSH | Base Sequence Binding Sites Blotting, Northern DNA Footprinting Electrophoretic Mobility Shift Assay Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Escherichia coli Proteins / physiology Ethylmaleimide / metabolism Gene Expression Regulation, Bacterial* Immunoblotting Models, Genetic Molecular Sequence Data Operon / genetics Oxidoreductases / genetics Oxidoreductases / metabolism* Oxidoreductases / physiology* Promoter Regions, Genetic / genetics Protein Binding Recombinant Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Transcription Factors / physiology |
| IF | 3.006 |
| 引用数 | 36 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JD22799 |