論文 - 詳細
| RRC ID | 35223 |
|---|---|
| 著者 | Karimova G, Robichon C, Ladant D. |
| タイトル | Characterization of YmgF, a 72-residue inner membrane protein that associates with the Escherichia coli cell division machinery. |
| ジャーナル | J Bacteriol |
| Abstract |
Formation of the Escherichia coli division septum is catalyzed by a number of essential proteins (named Fts) that assemble into a ring-like structure at the future division site. Many of these Fts proteins are intrinsic transmembrane proteins whose functions are largely unknown. In the present study, we attempted to identify a novel putative component(s) of the E. coli cell division machinery by searching for proteins that could interact with known Fts proteins. To do that, we used a bacterial two-hybrid system based on interaction-mediated reconstitution of a cyclic AMP (cAMP) signaling cascade to perform a library screening in order to find putative partners of E. coli cell division protein FtsL. Here we report the characterization of YmgF, a 72-residue integral membrane protein of unknown function that was found to associate with many E. coli cell division proteins and to localize to the E. coli division septum in an FtsZ-, FtsA-, FtsQ-, and FtsN-dependent manner. Although YmgF was previously shown to be not essential for cell viability, we found that when overexpressed, YmgF was able to overcome the thermosensitive phenotype of the ftsQ1(Ts) mutation and restore its viability under low-osmolarity conditions. Our results suggest that YmgF might be a novel component of the E. coli cell division machinery. |
| 巻・号 | 191(1) |
| ページ | 333-46 |
| 公開日 | 2009-1-1 |
| DOI | 10.1128/JB.00331-08 |
| PII | JB.00331-08 |
| PMID | 18978050 |
| PMC | PMC2612424 |
| MeSH | Adenylyl Cyclases / genetics Bacterial Proteins / genetics Bordetella pertussis / enzymology Bordetella pertussis / genetics Cell Division / physiology* Cloning, Molecular DNA Primers DNA, Bacterial / genetics Escherichia coli / cytology* Escherichia coli Proteins / genetics* Escherichia coli Proteins / physiology Gene Library Membrane Proteins / genetics* Membrane Proteins / physiology Plasmids Polymerase Chain Reaction / methods |
| IF | 3.006 |
| 引用数 | 39 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW1156-KC JW5449-KC JW5646-KC JW0729-KC JW0727-KC JW0731-KC |